Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Macro H2A.2.
(Peptide available as ab15023.)
Our Abpromise guarantee covers the use of ab4173 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This image is courtesy of Juan Ausio
CL = chicken liver and CE= chicken erythrocyte. Approximately 2 micrograms were loaded in each lane, blotted and reacted with primary antibody at dilution of 1:500 and 1:3000 for the secondary.
Previously, the problem has been that macroH2A2 is not very ubiquitous compared to macro H2A.1. While this makes it good for commercialization purposes it is hard to prepare chromatin fractions that are enriched in this fraction. However, these images show a ponceau stain (left hand side blot) and the western using the ab4173 antibody. There is one specific band.