Overview

  • Product name
  • Description
    Rabbit polyclonal to MACROD1
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: SFLKGLSDKQ REEHYFCKDF VRLKKIPTWK EMAKGVAVKV EEPRYKKDKQ LNEKISLLRS DITKLEV, corresponding to amino acids of Human MACROD1.

  • Positive control
    • Human colon tissue.

Applications

Our Abpromise guarantee covers the use of ab122688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Plays a role in estrogen signaling. Binds to androgen receptor (AR) and amplifies the transactivation function of AR in response to androgen. May play an important role in carcinogenesis and/or progression of hormone-dependent cancers by feed-forward mechanism that activates ESR1 transactivation. Could be an ESR1 coactivator, providing a positive feedback regulatory loop for ESR1 signal transduction. Could be involved in invasive growth by down-regulating CDH1 in endometrial cancer cells. Enhances ESR1-mediated transcription activity.
  • Involvement in disease
    Note=A chromosomal aberration involving MACROD1 is found in acute leukemia. Translocation t(11;21)(q13;q22) that forms a RUNX1-MACROD1 fusion protein.
  • Sequence similarities
    Contains 1 Macro domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • LRP16 antibody
    • MACD1_HUMAN antibody
    • MACRO domain containing 1 antibody
    • MACRO domain-containing protein 1 antibody
    • Macrod1 antibody
    • O-acetyl-ADP-ribose deacetylase MACROD1 antibody
    • Protein LRP16 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in mitochondria. Recommended concentration of ab122688 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122688, at 1/200 dilution, staining MACROD1 in paraffin-embedded Human colon tissue by Immunohistochemistry.

References

This product has been referenced in:
  • Ahmed S  et al. Loss of the Mono-ADP-ribosyltransferase, Tiparp, Increases Sensitivity to Dioxin-induced Steatohepatitis and Lethality. J Biol Chem 290:16824-40 (2015). Read more (PubMed: 25975270) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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