Overview

Description

  • Nature
    Synthetic
  • Amino Acid Sequence

    Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab37263 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Form
      Liquid
    • Additional notes

      - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
      - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
      - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
      - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
      - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      Information available upon request.

    General Info

    • Alternative names
      • Core histone macro h2a.1
      • Core histone macro-H2A.1
      • H2A histone family member Y
      • H2A.y
      • H2A/y
      • H2AF12M
      • H2AFJ
      • H2afy
      • H2AY_HUMAN
      • Histone H2A.Y
      • Histone macroH2A1
      • Histone macroH2A1.1
      • Histone macroH2A1.2
      • Macroh2a1
      • MACROH2A1.1
      • MacroH2A1.2
      • Medulloblastoma antigen MU MB 50.205
      • Medulloblastoma antigen MU-MB-50.205
      • mH2a
      • mH2A1
      see all
    • Function
      Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Contains 1 histone H2A domain.
      Contains 1 Macro domain.
    • Post-translational
      modifications
      Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin.
    • Cellular localization
      Nucleus. Chromosome. Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin.
    • Information by UniProt

    References

    ab37263 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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