Anti-Macrophage antibody [BM8] (Phycoerythrin) (ab125148)

Overview

  • Product nameAnti-Macrophage antibody [BM8] (Phycoerythrin)See all Macrophage primary antibodies ...
  • Description
    Rat monoclonal [BM8] to Macrophage (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsWB, IHC-P, IHC-Fr, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    BALB/c macrophages obtained from 14-day-old bone marrow cell cultures.

  • Positive control
    • Mouse liver tissue; Mouse macrophages and Human heart macrophages.

Properties

Applications

Our Abpromise guarantee covers the use of ab125148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 125 kDa.
IHC-P Use at an assay dependent dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent dilution. Use acetone fixation.
Flow Cyt Use at an assay dependent concentration. Use 10 µl per 250,000 cells in a 100 µl total staining volume.

Target

  • RelevanceMacrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becomming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognised as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).
  • Alternative names
    • macrophages antibody

Anti-Macrophage antibody [BM8] (Phycoerythrin) images

  • ab125148, staining Macrophages in a frozen section of Mouse liver by Immunohistochemistry.

References for Anti-Macrophage antibody [BM8] (Phycoerythrin) (ab125148)

This product has been referenced in:
  • Leenen PJ  et al. Markers of mouse macrophage development detected by monoclonal antibodies. J Immunol Methods 174:5-19 (1994). Read more (PubMed: 8083537) »

See 1 Publication for this product

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