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BALB/c macrophages obtained from 14-day-old bone marrow cell cultures.
Our Abpromise guarantee covers the use of ab125148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 125 kDa.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration. Use acetone fixation.|
|Flow Cyt||Use at an assay dependent concentration. Use 10 µl per 250,000 cells in a 100 µl total staining volume.
Ab134674-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"