PE Anti-Macrophage antibody [HIS36] (ab95574)
Key features and details
- PE Mouse monoclonal [HIS36] to Macrophage
- Suitable for: Flow Cyt
- Reacts with: Rat
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Overview
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Product name
PE Anti-Macrophage antibody [HIS36]
See all Macrophage primary antibodies -
Description
PE Mouse monoclonal [HIS36] to Macrophage -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Tissue, cells or virus corresponding to Rat Macrophage. Rat bone marrow cells.
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Positive control
- Rat spleen and peritoneal cells
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General notes
The HIS36 monoclonal antibody reacts with a molecule expressed on the surface of Rat macrophages. The antigen recognized by HIS36 is an ED2-like antigen expressed by thioglycolate elicited peritoneal cells and tissue macrophages. ab95574 is used as a marker for Rat tissue macrophages.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
The HIS36 monoclonal antibody reacts with a molecule expressed on the surface of Rat macrophages. The antigen recognized by HIS36 is an ED2-like antigen expressed by thioglycolate elicited peritoneal cells and tissue macrophages. ab95574 is used as a marker for Rat tissue macrophages. -
Clonality
Monoclonal -
Clone number
HIS36 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 0.25-0.5µg for 105-8 cells.
A final volume of 100 µL is recommended for staining the cell sample.
ab91363 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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Notes |
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Flow Cyt
Use 0.25-0.5µg for 105-8 cells. A final volume of 100 µL is recommended for staining the cell sample.
ab91363 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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Target
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Relevance
Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becomming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognised as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues). -
Alternative names
- macrophages antibody
Images
Datasheets and documents
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Datasheet download
References (0)
ab95574 has not yet been referenced specifically in any publications.