Anti-Macrophage antibody [HIS36] (Phycoerythrin) (ab95574)

Overview

  • Product name
    Anti-Macrophage antibody [HIS36] (Phycoerythrin)
    See all Macrophage primary antibodies
  • Description
    Mouse monoclonal [HIS36] to Macrophage (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat bone marrow cells.

  • Positive control
    • Rat spleen and peritoneal cells
  • General notes


    The HIS36 monoclonal antibody reacts with a molecule expressed on the surface of Rat macrophages. The antigen recognized by HIS36 is an ED2-like antigen expressed by thioglycolate elicited peritoneal cells and tissue macrophages. ab95574 is used as a marker for Rat tissue macrophages.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, 150mM Sodium chloride, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    The HIS36 monoclonal antibody reacts with a molecule expressed on the surface of Rat macrophages. The antigen recognized by HIS36 is an ED2-like antigen expressed by thioglycolate elicited peritoneal cells and tissue macrophages. ab95574 is used as a marker for Rat tissue macrophages.
  • Clonality
    Monoclonal
  • Clone number
    HIS36
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.25-0.5µg for 105-8 cells. A final volume of 100 µL is recommended for staining the cell sample.



ab91363-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becomming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognised as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).
  • Alternative names
    • macrophages antibody

Images

  • ab95574, at 0.25 µg per test, staining Rat thioglycolate induced peritoneal exudate cells (PECs) by flow cytometry (filled histogram). The open histogram shows staining with 0.25 µg of Mouse IgG2a ? Isotype Control PE. Total viable cells were used for analysis.

References

ab95574 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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