The HIS36 monoclonal antibody reacts with a molecule expressed on the surface of Rat macrophages. The antigen recognized by HIS36 is an ED2-like antigen expressed by thioglycolate elicited peritoneal cells and tissue macrophages. ab95574 is used as a marker for Rat tissue macrophages.
Storage instructionsShipped at 4°C. Store at +4°C.
Primary antibody notesThe HIS36 monoclonal antibody reacts with a molecule expressed on the surface of Rat macrophages. The antigen recognized by HIS36 is an ED2-like antigen expressed by thioglycolate elicited peritoneal cells and tissue macrophages. ab95574 is used as a marker for Rat tissue macrophages.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.25-0.5µg for 105-8 cells. A final volume of 100 µL is recommended for staining the cell sample.
ab91363-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
RelevanceMacrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becomming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognised as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).
ab95574, at 0.25 µg per test, staining Rat thioglycolate induced peritoneal exudate cells (PECs) by flow cytometry (filled histogram). The open histogram shows staining with 0.25 µg of Mouse IgG2a ? Isotype Control PE. Total viable cells were used for analysis.
References for Anti-Macrophage antibody [HIS36] (Phycoerythrin) (ab95574)
has not yet been referenced specifically in any publications.
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