Anti-Macrophage antibody [MAC387] (FITC) (ab7429)

Overview

  • Product nameAnti-Macrophage antibody [MAC387] (FITC)
    See all Macrophage primary antibodies
  • Description
    Mouse monoclonal [MAC387] to Macrophage (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityThis antibody recognises the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and tissue macrophages. Variable results have been reported for staining brain macrophages and microglia.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Human, Pig, Monkey, Baboon, Cynomolgus Monkey, Rhesus monkey
  • Immunogen

    Human monocytes

  • General notes

    Fluorescein isothiocyanate (FITC) isomer 1.

Properties

Applications

Our Abpromise guarantee covers the use of ab7429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells. Membrane permeabilisation is required for this application.

Target

  • RelevanceMacrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becomming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognised as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).
  • Alternative names
    • macrophages antibody

Anti-Macrophage antibody [MAC387] (FITC) images

  • ab7429 staining Macrophage (FITC) in human peripheral blood granulocyte by Flow Cytometry analysis.

References for Anti-Macrophage antibody [MAC387] (FITC) (ab7429)

This product has been referenced in:
  • Burwitz BJ  et al. Technical advance: liposomal alendronate depletes monocytes and macrophages in the nonhuman primate model of human disease. J Leukoc Biol 96:491-501 (2014). Read more (PubMed: 24823811) »
  • Gutierrez M  et al. The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques. Vet Immunol Immunopathol 71:321-34 (1999). Read more (PubMed: 10587310) »

See all 5 Publications for this product

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This antibody recognized the L1 or Calprotectin molecule which is expressed by granulocytes, monocytes and tissue macrophages. In staining samples by flow cytometry which contains all of these relevant populations...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Rat Cell (macrophages (peritoneal))
Specification macrophages (peritoneal)
Username

Mr. olivier Reynard

Verified customer

Submitted Jul 24 2006

After a careful search with the sources of our macrophage antibodies I regret to inform you that we have no products in our product range which can identify macrophage form monocyte by Western blot and are cross reactive with human. In our product ...

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This antibody has not yet been tested in frozen sections.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"