Overview

  • Product name
    Anti-Macrophage antibody [PM-2K]
    See all Macrophage primary antibodies
  • Description
    Mouse monoclonal [PM-2K] to Macrophage
  • Specificity
    Does not recognize Macrophage Scavenger Receptor AI or AII as determined using positive CHO cells.
  • Tested applications
    Suitable for: WB, IHC-Fr, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Cow, Cat, Dog, Human, Pig, Monkey
    Does not react with: Rat, Rabbit, Goat, Horse, Guinea pig, Hamster
  • Immunogen

    Cultured human peritoneal macrophages.

  • Positive control
    • Macrophage cell membranes.

Properties

Applications

Our Abpromise guarantee covers the use of ab58822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-Fr 1/200 - 1/800.
Flow Cyt Use a concentration of 2 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Relevance
      Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becomming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognised as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).
    • Alternative names
      • macrophages antibody

    References for Anti-Macrophage antibody [PM-2K] (ab58822)

    This product has been referenced in:
    • Marshall DC  et al. Selective Allosteric Inhibition of MMP9 Is Efficacious in Preclinical Models of Ulcerative Colitis and Colorectal Cancer. PLoS One 10:e0127063 (2015). IHC . Read more (PubMed: 25961845) »
    • Tsai YM  et al. Functional interaction of cockroach allergens and mannose receptor (CD206) in human circulating fibrocytes. PLoS One 8:e64105 (2013). Flow Cyt ; Human . Read more (PubMed: 23734186) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Permeabilization
    No
    Sample
    Human Cell (peripheral blood)
    Specification
    peripheral blood
    Gating Strategy
    Gated on CD45+PM2K+ cells
    Preparation
    Cell harvesting/tissue preparation method: The blood samples are centrifuged at 2500RPM for 10 mins, and lyzed with ACK buffer 2X, 5 mins each and suspended in FA buffer
    Sample buffer: FA buffer (1X PBS+0.2% FBS)
    Username

    Dr. Yunge Zhao

    Verified customer

    Submitted Jun 19 2013

    Thank you for contacting Abcam.

    The expected band size is around 150kDA. I am sorry to hear of the difficulties that you have been experiencing using these products. Would you mind sending me the details of your protocol including the tissue...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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