Anti-Macrophage antibody [RM0029-11H3] (ab56297)
Key features and details
- Rat monoclonal [RM0029-11H3] to Macrophage
- Suitable for: ICC/IF, Flow Cyt, IHC-P
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-Macrophage antibody [RM0029-11H3]
See all Macrophage primary antibodies -
Description
Rat monoclonal [RM0029-11H3] to Macrophage -
Host species
Rat -
Tested applications
Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Isolated mouse peritoneal macrophages
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Positive control
- Spleen, Lymph node, Diseased (GBM) mouse kidney tissue. IF/ICC: RAW246.7
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
RM0029-11H3 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab56297 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 10 µg/ml.
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Flow Cyt |
Use 2µg for 106 cells.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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IHC-P | (2) |
Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 10 µg/ml. |
Flow Cyt
Use 2µg for 106 cells. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
IHC-P
Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. |
Target
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Relevance
Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becomming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognised as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues). -
Alternative names
- macrophages antibody
Images
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ICC/IF image of ab56297 stained RAW246.7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56297, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96887, DyLight® 488 goat anti-rat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
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Bouin’s solution fixed and paraffin embedded mouse kidney section from anti-GBM model was subjected to immunohistochemistry staining (ABC) of Macrophage using ab56297.
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Ab56297 staining macrophage in mouse brain tumour tissue sections by (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 0.5% TNB blocking reagent for 30 minutes at 25°C. Samples were incubated with primary antibody at 1/100 dilution for 18 hours at 4 C. A goat anti-rat IgG H&L (HRP) (ab7097) was used at 1/500 dilution.
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Overlay histogram showing RAW 264.7 cells stained with ab56297 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56297, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758] (ab18450, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in RAW 264.7 cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.
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Immunohistochemical analysis of murine tumour tissue, staining Macrophage with ab56297. Antigen retrieval was performed under high pressure in 10 mM EDTA buffer (pH 8.0) before incubation with primary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (35)
ab56297 has been referenced in 35 publications.
- Steiner K & Humpel C Effects of Ischemia on the Migratory Capacity of Microglia Along Collagen Microcontact Prints on Organotypic Mouse Cortex Brain Slices. Front Cell Neurosci 16:858802 (2022). PubMed: 35783100
- Fang M et al. TRIM18 is a critical regulator of viral myocarditis and organ inflammation. J Biomed Sci 29:55 (2022). PubMed: 35909127
- Xiang X et al. Proximal Tubule p53 in Cold Storage/Transplantation-Associated Kidney Injury and Renal Graft Dysfunction. Front Med (Lausanne) 8:746346 (2021). PubMed: 34746182
- Ma Z et al. p53/microRNA-214/ULK1 axis impairs renal tubular autophagy in diabetic kidney disease. J Clin Invest 130:5011-5026 (2020). PubMed: 32804155
- Rasaei R et al. Regulation of JAM2 Expression in the Lungs of Streptozotocin-Induced Diabetic Mice and Human Pluripotent Stem Cell-Derived Alveolar Organoids. Biomedicines 8:N/A (2020). PubMed: 32932992