Anti-Macrophage antibody [SPM281], prediluted (ab75693)

Overview

  • Product nameAnti-Macrophage antibody [SPM281], prediluted
    See all Macrophage primary antibodies
  • Description
    Mouse monoclonal [SPM281] to Macrophage, prediluted
  • SpecificityThis antibody has been shown as an important marker for identifying macrophages in tissue sections. It reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia.
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Cat, Dog, Human, Pig, Monkey, Baboon
  • Immunogen

    Affinity purified monocyte membrane preparation

  • Positive control
    • Human tonsil, lymph node or spleen tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab75693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: Ready to use for 30 minutes at room temperature.
    For staining of formalin tissues, digest sections with trypsin at 1mg/ml PBS, 10 minutes at 37oC.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceMacrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becomming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognised as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).
    • Alternative names
      • macrophages antibody

    Anti-Macrophage antibody [SPM281], prediluted images

    • ab75693, prediluted, staining Macrophage in formalin fixed, paraffin embedded human tonsil tissue by Immunohistochemistry.

    References for Anti-Macrophage antibody [SPM281], prediluted (ab75693)

    This product has been referenced in:
    • Bouari C  et al. Antimicrobial activity of Mentha piperita and Saturenja hortensis in a murine model of cutaneous protothecosis. J Mycol Med 24:34-43 (2014). Mouse . Read more (PubMed: 24387811) »
    • Zheng Y  et al. Microstructured templates for directed growth and vascularization of soft tissue in vivo. Biomaterials 32:5391-401 (2011). IHC-P ; Mouse . Read more (PubMed: 21549426) »

    See all 2 Publications for this product

    Product Wall

    1. Following deparaffinization and rehydration, digest sections using trypsin at 1 mg/ml for 10 min at 37C. The primary antibody is incubated for 20 min at RT on tonsil. Detection is performed using a polymer based detection system with DAB.
    2. ...

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    Thank you for your email.
    I would recommed one of the following trypsin products:
    ab970: Trypsin Enzymatic Antigen Retrieval Solution
    http://www.abcam.com/trypsin-enzymatic-antigen-retrieval-solution-ab970.html
    ab64205: Trypsin Enzy...

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    Thank you for your email.
    Yes, this antibody would be a good choice and it would guaranteed under our Abpromise as rat and IHC-P have been tested with this antibody.
    Please see the datasheet for the IHC-P image with human tonsil tissue:
    ...

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    Thank you for your reply and for your patience while I have been in touch with the lab. We don't recommend adding serum as it will further dilute the antibody, but a separate serum blocking step should be sufficient. The prediluted solution is 4 ug/mL....

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    Thank you for contacting us. The concentration of Anti-Macrophage antibody [SPM281], prediluted (ab75693) is 4μg/mL. If you need any further information please do not hesitate to contact us again, in the meantime I wish you luck with your resea...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"