Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody (ab25128)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab25128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 11 kDa.
IP 1/500.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionMonokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C C motif chemokine 3 antibody
    • CCL 3 antibody
    • CCL3 antibody
    • CCL3_HUMAN antibody
    • Chemokine C C motif ligand 3 antibody
    • G0/G1 switch regulatory protein 19 1 antibody
    • G0/G1 switch regulatory protein 19-1 antibody
    • G0S19 1 antibody
    • G0S19 1 protein antibody
    • Heparin binding chemotaxis protein antibody
    • L2G25B antibody
    • LD78 alpha antibody
    • LD78-alpha(4-69) antibody
    • LD78alpha antibody
    • Macrophage inflammatory protein 1 alpha antibody
    • Macrophage inflammatory protein 1-alpha antibody
    • MIP 1 alpha antibody
    • MIP 1A antibody
    • MIP-1-alpha antibody
    • MIP-1-alpha(4-69) antibody
    • MIP1A antibody
    • PAT 464.1 antibody
    • SCYA 3 antibody
    • SCYA3 antibody
    • SIS alpha antibody
    • SIS beta antibody
    • SIS-beta antibody
    • Small inducible cytokine A3 antibody
    • small inducible cytokine A3 (homologous to mouse Mip-1a) antibody
    • Small-inducible cytokine A3 antibody
    • Tonsillar lymphocyte LD78 alpha protein antibody
    see all

Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody images

  • All lanes : Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody (ab25128)

    Lane 1 : RAW264.7 cell lysate treated with LPS (15 h).
    Lane 2 : RAW264.7 cell supernatant treated with LPS (15 h).


    Predicted band size : 11 kDa

References for Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody (ab25128)

This product has been referenced in:
  • Rantakari P  et al. Stabilin-1 expression defines a subset of macrophages that mediate tissue homeostasis and prevent fibrosis in chronic liver injury. Proc Natl Acad Sci U S A 113:9298-303 (2016). IHC-Fr ; Mouse . Read more (PubMed: 27474165) »
  • Wong L  et al. Experimental autoimmune prostatitis induces microglial activation in the spinal cord. Prostate 75:50-9 (2015). Read more (PubMed: 25263093) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us. The cells tested were fixed in 4% PFA and then permeabilized with Triton-X.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (RAW 264.7)
Specification RAW 264.7
Fixative Formaldehyde
Permeabilization Yes - 1% Triton-X
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Katrinb

Verified customer

Submitted Jul 02 2012

DISCOUNT CODE: **********
Expiration date: *

I am very pleased to hear you would like to accept our offer and test ab25128 in IF. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"