Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody (ab9781)

Overview

  • Product nameAnti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody
    See all Macrophage Inflammatory Protein 1 alpha / CCL3 primary antibodies
  • Description
    Rabbit polyclonal to Macrophage Inflammatory Protein 1 alpha / CCL3
  • Tested applicationsSuitable for: Indirect ELISA, WB, ELISA, Neutralising, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Highly pure (>98%) recombinant rMIP-1-alpha (Rat macrophage inflammatory protein-1 alpha)

Properties

Applications

Our Abpromise guarantee covers the use of ab9781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA
WB
ELISA
Neutralising
IHC-Fr
  • Application notesNeutralization:To yield one-half maximal inhibition [ND50] of the biological activity of rMIP-1a (100 ng/ml), a concentration of 8.0 - 10.0 µg/ml of this antibody is required.

    ELISA:To detect rMIP-1-alpha by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rMIP-1-alpha.

    Western Blot:To detect rMIP-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rMIP-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  • Target

    • FunctionMonokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
    • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
    • Post-translational
      modifications
      N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • C C motif chemokine 3 antibody
      • CCL 3 antibody
      • CCL3 antibody
      • CCL3_HUMAN antibody
      • Chemokine C C motif ligand 3 antibody
      • G0/G1 switch regulatory protein 19 1 antibody
      • G0/G1 switch regulatory protein 19-1 antibody
      • G0S19 1 antibody
      • G0S19 1 protein antibody
      • Heparin binding chemotaxis protein antibody
      • L2G25B antibody
      • LD78 alpha antibody
      • LD78-alpha(4-69) antibody
      • LD78alpha antibody
      • Macrophage inflammatory protein 1 alpha antibody
      • Macrophage inflammatory protein 1-alpha antibody
      • MIP 1 alpha antibody
      • MIP 1A antibody
      • MIP-1-alpha antibody
      • MIP-1-alpha(4-69) antibody
      • MIP1A antibody
      • PAT 464.1 antibody
      • SCYA 3 antibody
      • SCYA3 antibody
      • SIS alpha antibody
      • SIS beta antibody
      • SIS-beta antibody
      • Small inducible cytokine A3 antibody
      • small inducible cytokine A3 (homologous to mouse Mip-1a) antibody
      • Small-inducible cytokine A3 antibody
      • Tonsillar lymphocyte LD78 alpha protein antibody
      see all

    Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody images

    • Indirect ELISA measuring Macrophage Inflammatory Protein 1 alpha / CCL3 levels using ab9781.

      Supernatants collected from rat cerebral cortical cells after various treatments were coated onto 96-well flat bottom plates and incubated overnight at 4°C. The plates were blocked with 1% BSA at 37°C for 45 min before incubation with primary antibody (1/1000) in bicarbonate buffer overnight at 4°C. An HRP-conjugated secondary IgG (1/1000) was added and incubated for 45 min at 37°C, in the dark. The reaction was developed by adding o-phenylene diamine H2O2 for 20 min. Optical density was measured at 450 nm.

    References for Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody (ab9781)

    This product has been referenced in:
    • Johnson EA  et al. Increased expression of the chemokines CXCL1 and MIP-1a by resident brain cells precedes neutrophil infiltration in the brain following prolonged soman-induced status epilepticus in rats. J Neuroinflammation 8:41 (2011). IHC-Fr ; Rat . Read more (PubMed: 21535896) »
    • Tripathy D & Grammas P Acetaminophen protects brain endothelial cells against oxidative stress. Microvasc Res 77:289-96 (2009). ELISA ; Rat . Read more (PubMed: 19265712) »

    See all 4 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Tissue lysate - whole (liver)
    Loading amount 30 µg
    Specification liver
    Treatment LPS 1 hour
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. Haoshu Fang

    Verified customer

    Submitted Nov 19 2012

    Thank you for contacting Abcam.

    All of the antibodies that you mentioned are guaranteed under our Abpromise to work in a Neutralizing assay, in various species:

    ab89546 - Works human samples
    ab9673 – Works in human samples Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"