Recombinant
RabMAb

Anti-Macrophage inflammatory protein 1 alpha/CCL3+CCL3L1 antibody [EP493Y] (ab46786)

Overview

  • Product name
    Anti-Macrophage inflammatory protein 1 alpha/CCL3+CCL3L1 antibody [EP493Y]
  • Description
    Rabbit monoclonal [EP493Y] to Macrophage inflammatory protein 1 alpha/CCL3+CCL3L1
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Macrophage inflammatory protein 1 alpha/CCL3+CCL3L1 aa 50 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P10147

  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab46786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Anti-Macrophage inflammatory protein 1 alpha/CCL3+CCL3L1 antibody [EP493Y] images

    • All lanes : Anti-Macrophage inflammatory protein 1 alpha/CCL3+CCL3L1 antibody [EP493Y] (ab46786) at 1/2000 dilution

      Lane 1 : Human Macrophage Inflammatory Protein 1 alpha / CCL3 active protein
      Lane 2 : Human Macrophage Inflammatory protein 1 beta / CCL4 active protein
      Lane 3 : Human Macrophage Inflammatory active protein 4/CCL18
      Lane 4 : Human CCL3L1 recombinant protein

      Lysates/proteins at 0.02 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size : 10 kDa
      Observed band size : kDa (why is the actual band size different from the predicted?)


      Exposure time : 10 seconds

      Human CCL3 active protein contains aa27-92.

      Human CCL4 active protein contains aa24-92.

      Human CCL18 active protein contains aa21-89.

      Human CCL3L1 recombinant protein contain 2*(aa28-93) sequence with His-tag and GST-tag.

      Blocking and diluting buffer: 5% NFDM/TBST.

    • All lanes : Anti-Macrophage inflammatory protein 1 alpha/CCL3+CCL3L1 antibody [EP493Y] (ab46786) at 1/1000 dilution

      Lane 1 : Untreated THP-1 (Human monocytic leukemia cell line) lysate
      Lane 2 : THP-1 lysate treated with 100 nM Phorbol-12-myristate-13-acetate (PMA) overnight, then treated with Lipopolysaccharides (LPS) at 100 ng/ml for 4 hour and then 1 µg/ml Brefeldin A was added for the last 3 hours.

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size : 10 kDa
      Observed band size : 10 kDa


      Exposure time : 3 minutes

      Blocking and diluting buffer: 5% NFDM/TBST.

      Macrophage inflammatory protein 1 alpha is induced in macrophages following exposure to bacterial lipopolysaccharide (LPS), PMID: 9848081.

    References for Anti-Macrophage inflammatory protein 1 alpha/CCL3+CCL3L1 antibody [EP493Y] (ab46786)

    ab46786 has not yet been referenced specifically in any publications.

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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