Anti-Macrophage Inflammatory Protein 1 beta antibody (ab10386)

Overview

  • Product nameAnti-Macrophage Inflammatory Protein 1 beta antibody
    See all Macrophage Inflammatory Protein 1 beta primary antibodies
  • Description
    Goat polyclonal to Macrophage Inflammatory Protein 1 beta
  • SpecificityMouse MIP1 beta neutralizes the bioactivity of recombinant, mouse MIP1 beta, but not that of recombinant, human MIP1 beta, recombinant, human MIP1 alpha or recombinant, mouse MIP1 alpha. In indirect ELISA and immunoblotting, this antibody displays less than 2% cross-reactivity with recombinant, mouse MIP1 alpha, recombinant, human MIP1 alpha and recombinant, human MIP1 alpha. The product shows no cross-reactivity with other tested cytokines using indirect ELISA.
  • Tested applicationsSuitable for: ELISA, WB, Neutralisingmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse recombinant macrophage inflammatory protein-1beta, expressed in E. coli, as the immunogen.

  • General notesEndotoxin: <10 ng/vial.

Properties

Applications

Our Abpromise guarantee covers the use of ab10386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
Neutralising
  • Application notesELISA: Use at a concentration of 0.5 µg/ml to detect <1ng/well of recombinant mouse MIP1 beta.
    Neut: Use at a concentration of 50 - 100 µg/ml.
    WB: Use at a concentration of 1 µg/ml to detect recombinant mouse MIP1 beta at 5ng/lane under reducing conditions.
    Predicted molecular weight: 11 kDa.

    Anti-Mouse MIP1 beta was tested for its ability to neutralize the bioactivity of recombinant, mouse MIP1 beta in an in vitro colony forming assay using hematopoietic stem cells.

    The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of recombinant, mouse MIP1 beta, which is present at five times its own EC50 (the concentration of recombinant, mouse MIP1 beta producing a one-half maximal bioactivity without antibody).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMonokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
    • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
    • Post-translational
      modifications
      N-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • MIP 1 beta antibody
      • Secreted protein G 26 antibody
      • ACT 2 antibody
      • ACT-2 antibody
      • ACT2 antibody
      • AT744.1 antibody
      • AT744.2 antibody
      • C C motif chemokine 4 antibody
      • C C motif chemokine 4 like antibody
      • C C motif chemokine ligand 4 like 1 antibody
      • CC chemokine ligand 4 antibody
      • CC chemokine ligand 4L1 antibody
      • CC chemokine ligand 4L1d2 antibody
      • CC chemokine ligand 4L2 antibody
      • CCL4 antibody
      • CCL4_HUMAN antibody
      • ccl4l 1 antibody
      • CCL4L antibody
      • CCL4L1 antibody
      • Chemokine (C C motif) ligand 4 antibody
      • Chemokine (C C motif) ligand 4 like 1 antibody
      • Chemokine (C C motif) ligand 4 like 1, telomeric antibody
      • Chemokine (C C motif) ligand 4 like 2 antibody
      • Chemokine CC Motif Ligand 4 antibody
      • G 26 antibody
      • G 26 T lymphocyte secreted protein antibody
      • G-26 T-lymphocyte-secreted protein antibody
      • HC21 antibody
      • Immune activation 2 antibody
      • LAG 1 antibody
      • LAG-1 antibody
      • LAG1 antibody
      • Lymphocyte activation gene 1 antibody
      • Lymphocyte activation gene 1 protein antibody
      • Macrophage inflammatory protein 1 beta antibody
      • Macrophage inflammatory protein 1-beta antibody
      • Macrophage inflammatory protein 1b2 antibody
      • MGC104418 antibody
      • MGC126025 antibody
      • MGC126026 antibody
      • MIP-1-beta antibody
      • MIP-1-beta(1-69) antibody
      • MIP-1-beta(3-69) antibody
      • MIP1 beta antibody
      • MIP1B antibody
      • MIP1B1 antibody
      • Monocyte adherence induced protein 5 alpha antibody
      • PAT 744 antibody
      • Protein H400 antibody
      • SCYA2 antibody
      • SCYA4 antibody
      • SCYA4L antibody
      • SCYA4L1 antibody
      • SCYQ4L2 antibody
      • Secreted protein G 26 antibody
      • Secreted protein G26 antibody
      • SIS gamma antibody
      • SIS-gamma antibody
      • Small inducible cytokine A4 (homologous to mouse Mip 1b) antibody
      • Small inducible cytokine A4 antibody
      • small inducible cytokine A4-like antibody
      • Small-inducible cytokine A4 antibody
      • T cell activation protein 2 antibody
      • T-cell activation protein 2 antibody
      see all

    References for Anti-Macrophage Inflammatory Protein 1 beta antibody (ab10386)

    This product has been referenced in:
    • Johannsen A  et al. Definition of Key Variables for the Induction of Optimal NY-ESO-1-Specific T Cells in HLA Transgene Mice. J Immunol 185:3445-55 (2010). Neutralising ; Mouse . Read more (PubMed: 20733200) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"