Recombinant Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (ab40857)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR494] to CCL4/MIP-1 beta + CCL4L1
- Suitable for: IP, ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] -
Description
Rabbit monoclonal [EPR494] to CCL4/MIP-1 beta + CCL4L1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, ICC/IF, WBmore details
Unsuitable for: IHC -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CCL4/MIP-1 beta aa 50 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P13236 -
Positive control
- THP-1 cell lysate
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR494 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
1/50.
|
|
ICC/IF |
1/100.
|
|
WB |
1/1000 - 1/500000. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa).
|
Notes |
---|
IP
1/50. |
ICC/IF
1/100. |
WB
1/1000 - 1/500000. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa). |
Target
-
Cellular localization
CCL4/MIP-1 beta: Secreted. CCL4L1: Secreted. -
Database links
- Entrez Gene: 388372 Human
- Entrez Gene: 6351 Human
- Entrez Gene: 9560 Human
- Omim: 182284 Human
- Omim: 603782 Human
- Omim: 610757 Human
- SwissProt: P13236 Human
- SwissProt: Q8NHW4 Human
see all
Images
-
All lanes : Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (ab40857) at 1/1000 dilution
Lane 1 : Untreated THP-1 (human acute monocytic leukemia) lysate
Lane 2 : THP-1 (human acute monocytic leukemia) treated with 100 nM Phorbol-12-myristate-13-acetate(PMA) overnight, then treated with Lipopolysaccharides (LPS) 100 ng/mL for 7 hours and then 1 µg/mL Brefeldin A was added for the last 3 hours, lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 10 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Diluting buffer 5% NFDM /TBST.
CCL4/MIP-1 beta (CCL4) is induced in macrophages following exposure to bacterial LPS (PMID: 9848081).
-
Immunocytochemistry/Immunofluorescence analysis of THP-1 (Human monocytic leukemia cell line) labeling CCL4/MIP-1 beta/CCL4 + CCL4L with ab40857 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200. Nuclei were counterstained with DAPI (blue).
The expression increased after treatment with Lipopolysaccharides (LPS), 100 ng/mL for 4 hours, followed by addition of Brefeldin A (1 μg/mL) for 3 hours.
-
All lanes : Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (ab40857) at 1/10000 dilution
Lane 1 : Untagged human CCL4 recombinant protein (aa24-92)
Lane 2 : Untagged human CCL4L recombinant protein (aa24-92)
Lane 3 : GST-tagged human CCL3 recombinant protein (aa27-92)
Lane 4 : GST-tagged human CCL3L recombinant protein 2*(aa28-93)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 10 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking/Diluting buffer 5% NFDM /TBST
-
ab40857 at 1/50 immunoprecipitating CCL4/MIP-1 beta/CCL4 + CCL4L in THP-1 (Human monocytic leukemia cell line) whole cell lysate observed at 12 KDa (lanes 1 and 2).
Lane 1 (input): THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/mL LPS for 7 hours and 1 μg/mL Brefeldin A was added for the last 3 hours whole cell lysate, 10μg
Lane 2 (+): ab40857 + THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/mL LPS for 7 hours and 1μg/mL Brefeldin A was added for the last 3 hours whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40857 in THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/mL LPS for 7 hours and 1 μg/mL Brefeldin A was added for the last 3 hours whole cell lysate
For western blotting: ab40857 at 1/1000 followed by ab131366 VeriBlot for IP Detection Reagent (HRP) for detection at 1/000.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab40857 has not yet been referenced specifically in any publications.