Recombinant
RabMAb

Anti-Macrophage Inflammatory Protein 1 beta antibody [EP521Y] (ab45690)

Overview

  • Product name
    Anti-Macrophage Inflammatory Protein 1 beta antibody [EP521Y]
    See all Macrophage Inflammatory Protein 1 beta primary antibodies
  • Description
    Rabbit monoclonal [EP521Y] to Macrophage Inflammatory Protein 1 beta
  • Tested applications
    Suitable for: IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    corresponding to Human Macrophage Inflammatory Protein 1 beta aa 1 to the C-terminus (N terminal).
    Database link: P13236

  • Positive control
    • THP-1, Raw264.7
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab45690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/60.
WB 1/1000 - 1/500000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
ICC/IF 1/100.

Target

  • Function
    Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    N-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • MIP 1 beta antibody
    • Secreted protein G 26 antibody
    • ACT 2 antibody
    • ACT-2 antibody
    • ACT2 antibody
    • AT744.1 antibody
    • AT744.2 antibody
    • C C motif chemokine 4 antibody
    • C C motif chemokine 4 like antibody
    • C C motif chemokine ligand 4 like 1 antibody
    • C C motif chemokine ligand 4 like 2 antibody
    • CC chemokine ligand 4 antibody
    • CC chemokine ligand 4L1 antibody
    • CC chemokine ligand 4L1d2 antibody
    • CC chemokine ligand 4L2 antibody
    • CCL4 antibody
    • CCL4_HUMAN antibody
    • ccl4l 1 antibody
    • CCL4L antibody
    • CCL4L1 antibody
    • Chemokine (C C motif) ligand 4 antibody
    • Chemokine (C C motif) ligand 4 like 1 antibody
    • Chemokine (C C motif) ligand 4 like 1, telomeric antibody
    • Chemokine (C C motif) ligand 4 like 2 antibody
    • Chemokine CC Motif Ligand 4 antibody
    • G 26 antibody
    • G 26 T lymphocyte secreted protein antibody
    • G-26 T-lymphocyte-secreted protein antibody
    • HC21 antibody
    • Immune activation 2 antibody
    • LAG 1 antibody
    • LAG-1 antibody
    • LAG1 antibody
    • Lymphocyte activation gene 1 antibody
    • Lymphocyte activation gene 1 protein antibody
    • Macrophage inflammatory protein 1 beta antibody
    • Macrophage inflammatory protein 1-beta antibody
    • Macrophage inflammatory protein 1b2 antibody
    • MGC104418 antibody
    • MGC126025 antibody
    • MGC126026 antibody
    • MIP-1-beta antibody
    • MIP-1-beta(1-69) antibody
    • MIP-1-beta(3-69) antibody
    • MIP1 beta antibody
    • MIP1B antibody
    • MIP1B1 antibody
    • Monocyte adherence induced protein 5 alpha antibody
    • PAT 744 antibody
    • Protein H400 antibody
    • SCYA2 antibody
    • SCYA4 antibody
    • SCYA4L antibody
    • SCYA4L1 antibody
    • SCYA4L2 antibody
    • SCYQ4L2 antibody
    • Secreted protein G 26 antibody
    • Secreted protein G26 antibody
    • SIS gamma antibody
    • SIS-gamma antibody
    • Small inducible cytokine A4 (homologous to mouse Mip 1b) antibody
    • Small inducible cytokine A4 antibody
    • small inducible cytokine A4-like antibody
    • Small-inducible cytokine A4 antibody
    • T cell activation protein 2 antibody
    • T-cell activation protein 2 antibody
    see all

Images

  • All lanes : Anti-Macrophage Inflammatory Protein 1 beta antibody [EP521Y] (ab45690) at 1/1000 dilution

    Lane 1 : Untagged human CCL4 recombinant protein (aa24-92)
    Lane 2 : Untagged human CCL4L recombinant protein (aa24-92)
    Lane 3 : GST-tagged human CCL3 recombinant protein (aa27-92)
    Lane 4 : GST-tagged human CCL3L recombinant protein 2*(aa28-93)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 10 kDa
    Observed band size : 12 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 seconds

    Blocking/Diluting buffer and concentration 5% NFDM /TBST 

  • Immunocytochemistry/Immunofluorescence analysis of THP-1 (Human monocytic leukemia cell line) cells labeling Macrophage Inflammatory Protein 1 beta/CCL4 + CCL4L with ab45690 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291, ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution. DAPI was used to stain nuclei blue.

    The expression increased after treatment with Lipopolysaccharides (LPS), 100 ng/mL for 4 hours, followed by addition of Brefeldin A (1 μg/mL) for 3 hours.

  • All lanes : Anti-Macrophage Inflammatory Protein 1 beta antibody [EP521Y] (ab45690) at 1/1000 dilution

    Lane 1 : Untreated THP-1 (human acute monocytic leukemia) lysate
    Lane 2 : THP-1 treated with 100 nM Phorbol-12-myristate-13-acetate(PMA) overnight, then treated with Lipopolysaccharides (LPS) 100 ng/mL for 7 hours and then 1 µg/mL Brefeldin A was added for the last 3 hours, lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 100000

    Predicted band size : 10 kDa
    Observed band size : 12 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    Blocking/Diluting buffer and concentration 5% NFDM /TBST.

    Macrophage inflammatory protein 1 beta (CCL4) is induced in macrophages following exposure to bacterial LPS (PMID: 9848081).

  • ab45690 at 1/60 immunoprecipitating Macrophage Inflammatory Protein 1 beta/CCL4 + CCL4L in THP-1 (Human monocytic leukemia cell line) whole cell lysate observed at 12 KDa (lanes 1 and 2).

    Lane 1 (input): THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/mL LPS for 7 hours and 1 μg/mL Brefeldin A was added for the last 3 hours whole cell lysate, 10μg

    Lane 2 (+): ab45690 + THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/mL LPS for 7 hours and 1 μg/mL Brefeldin A was added for the last 3 hours whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab45690 in THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/mL LPS for 7 hours and 1 μg/mL Brefeldin A was added for the last 3 hours whole cell lysate

    For western blotting, ab45690 at 1/1000 and ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/1000).

    Blocking/Diluting buffer 5% NFDM/TBST

  • All lanes : Anti-Macrophage Inflammatory Protein 1 beta antibody [EP521Y] (ab45690) at 1/1000 dilution

    Lane 1 : Untreated Raw264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate
    Lane 2 : Raw264.7 (mouse abelson murine leukemia virus-induced tumor) treated with LPS 10µg/mL for 4 hours and then 1 µg/mL Brefeldin A was added for the last 3 hours lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 10 kDa


    Exposure time : 3 minutes

    Blocking/Diluting buffer and concentration 5% NFDM /TBST 

References

This product has been referenced in:
  • Zsiros E  et al. The Ovarian Cancer Chemokine Landscape Is Conducive to Homing of Vaccine-Primed and CD3/CD28-Costimulated T Cells Prepared for Adoptive Therapy. Clin Cancer Res 21:2840-50 (2015). Read more (PubMed: 25712684) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab45690.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up