The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. PubMed: 20799334
Use at an assay dependent dilution. To detect mMIP-1g by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMIP-1g.
Use at an assay dependent dilution. To detect mMIP-1g by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-1g is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
FunctionMonokine with inflammatory, pyrogenic and chemokinetic properties. Circulates at high concentrations in the blood of healthy animals. Binding to a high-affinity receptor activates calcium release in neutrophils. It also inhibits colony formation of bone marrow myeloid immature progenitors.
Tissue specificityExpressed mainly in the liver, lung, and the thymus, although some expression has been detected in a wide variety of tissues except brain.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.