Anti-Macrophage Inflammatory Protein 3 alpha antibody (ab9829)
Key features and details
- Rabbit polyclonal to Macrophage Inflammatory Protein 3 alpha
- Suitable for: IHC-P, ICC, ICC/IF, IHC-FoFr, WB, ELISA, Neutralising, Sandwich ELISA
- Reacts with: Mouse, Rat, Human
- Isotype: unknown
Overview
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Product name
Anti-Macrophage Inflammatory Protein 3 alpha antibody
See all Macrophage Inflammatory Protein 3 alpha primary antibodies -
Description
Rabbit polyclonal to Macrophage Inflammatory Protein 3 alpha -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, ICC/IF, IHC-FoFr, WB, ELISA, Neutralising, Sandwich ELISAmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human Macrophage Inflammatory Protein 3 alpha. Highly pure (>98%) recombinant hMIP-3-alpha (human macrophage inflammatory protein-3 alpha)
Database link: P78556 -
Positive control
- IHC-P: Hepatocellular carcinoma and colon cancer tissues.
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General notes
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab224188.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. The reconstituted antibody is stable for at least 2 weeks at 2-8C and at least 6 months at -20C -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
No preservative, sterile filtered -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (2) |
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
ICC |
Use at an assay dependent concentration. PubMed: 24633013
|
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ICC/IF |
Use at an assay dependent concentration. PubMed: 20952674
|
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IHC-FoFr |
Use at an assay dependent concentration. PubMed: 19305396
|
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 10 kDa.
To detect hMIP-3-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-3-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|
ELISA |
Use at an assay dependent concentration.
To detect hMIP-3-alpha by direct ELISA (using 100ul/well antibody solution) a concentration of at least 0.5ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-3-alpha. |
|
Neutralising |
Use at an assay dependent concentration.
To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-3-alpha (100 ng/ml), a concentration of 15.0 - 18.0 ug/ml of this antibody is required. |
|
Sandwich ELISA |
Use at an assay dependent concentration.
To detect Human MIP-3α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab271284 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIP-3α. |
Notes |
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IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC
Use at an assay dependent concentration. PubMed: 24633013 |
ICC/IF
Use at an assay dependent concentration. PubMed: 20952674 |
IHC-FoFr
Use at an assay dependent concentration. PubMed: 19305396 |
WB
Use at an assay dependent concentration. Predicted molecular weight: 10 kDa. To detect hMIP-3-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-3-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
ELISA
Use at an assay dependent concentration. To detect hMIP-3-alpha by direct ELISA (using 100ul/well antibody solution) a concentration of at least 0.5ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-3-alpha. |
Neutralising
Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-3-alpha (100 ng/ml), a concentration of 15.0 - 18.0 ug/ml of this antibody is required. |
Sandwich ELISA
Use at an assay dependent concentration. To detect Human MIP-3α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab271284 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIP-3α. |
Target
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Function
Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. -
Tissue specificity
Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsC-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6364 Human
- Entrez Gene: 20297 Mouse
- Entrez Gene: 29538 Rat
- Omim: 601960 Human
- SwissProt: P78556 Human
- SwissProt: O89093 Mouse
- SwissProt: P97884 Rat
- Unigene: 75498 Human
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Alternative names
- Beta chemokine exodus 1 antibody
- Beta-chemokine exodus-1 antibody
- C C motif chemokine ligand 20 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling Macrophage Inflammatory Protein 3 alpha with ab9829 at 4 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue labelling Macrophage Inflammatory Protein 3 alpha with ab9829 at 4 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
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ab9829 detecting Macrophage Inflammatory Protein 3 alpha using sandwich ELISA
Protocols
Datasheets and documents
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Datasheet download
References (37)
ab9829 has been referenced in 37 publications.
- Dajnoki Z et al. Primary alterations during the development of hidradenitis suppurativa. J Eur Acad Dermatol Venereol 36:462-471 (2022). PubMed: 34724272
- Luo Y et al. Keratin 17 Promotes T Cell Response in Allergic Contact Dermatitis by Upregulating C-C Motif Chemokine Ligand 20. Front Immunol 13:764793 (2022). PubMed: 35178048
- Xie T et al. CircSMARCC1 facilitates tumor progression by disrupting the crosstalk between prostate cancer cells and tumor-associated macrophages via miR-1322/CCL20/CCR6 signaling. Mol Cancer 21:173 (2022). PubMed: 36045408
- Yamane T et al. Iron accelerates Fusobacterium nucleatum-induced CCL8 expression in macrophages and is associated with colorectal cancer progression. JCI Insight 7:N/A (2022). PubMed: 36136589
- Mandal DP et al. A Plausible Proposition of CCL20-Related Mechanism in Fusobacterium nucleatum-Associated Oral Carcinogenesis. Life (Basel) 11:N/A (2021). PubMed: 34833094