Anti-Macrophage Inflammatory Protein 3 antibody (ab9929)

Overview

  • Product nameAnti-Macrophage Inflammatory Protein 3 antibody
    See all Macrophage Inflammatory Protein 3 primary antibodies
  • Description
    Rabbit polyclonal to Macrophage Inflammatory Protein 3
  • Tested applicationsSuitable for: WB, ELISA, Neutralisingmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hMIP-3 (human macrophage inflammatory protein-3)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab9929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
Neutralising
  • Application notesNeutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-3 (100 ng/ml), a concentration of 2.0 - 4.0 µg/ml of this antibody is required.

    ELISA:To detect hMIP-3 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-3.

    Western Blot:To detect hMIP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  • Target

    • FunctionShows chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23.
    • Tissue specificityHigh levels in adult lung, liver, skeletal muscle and pancreas. Moderate levels in fetal liver, adult bone marrow and placenta. The short form is the major species and the longer form was detected only in very low abundance. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are found in high levels in synovial fluids from rheumatoid patients.
    • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • C-C motif chemokine 23 antibody
      • C6 beta chemokine antibody
      • CCL 23 antibody
      • CCL23 antibody
      • CCL23(30-99) antibody
      • CCL23_HUMAN antibody
      • Chemokine CC motif ligand 23 antibody
      • ck beta 8 antibody
      • CK-beta-8 antibody
      • CKB 8 antibody
      • CKB-8 antibody
      • Ckb-8-1 antibody
      • CKB8 antibody
      • Hmrp-2a antibody
      • Macrophage inflammatory protein 3 antibody
      • MIP 3 antibody
      • MIP-3 antibody
      • MIP3 antibody
      • MPIF 1 antibody
      • MPIF-1 antibody
      • MPIF1 antibody
      • Myeloid progenitor inhibitory factor 1 antibody
      • OTTHUMP00000163948 antibody
      • SCYA 23 antibody
      • SCYA23 antibody
      • Small inducible cytokine A 23 antibody
      • Small inducible cytokine subfamily A (Cys-Cys), member 23 antibody
      • Small inducible cytokine subfamily A, member 23 antibody
      • Small-inducible cytokine A23 antibody
      see all

    References for Anti-Macrophage Inflammatory Protein 3 antibody (ab9929)

    ab9929 has not yet been referenced specifically in any publications.

    Product Wall

    This antibody was raised against our protein MIP-3, this is a 99aa therefore I think it will only recognise 1-99.This is the amino acid sequence: RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESY FETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTR...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"