Synthetic peptide designed within residues: PQKFIVDYSE TSPQCPKPGV ILLTKRGRQI CADPNKKWVQ KYISDLKLNA, corresponding to internal sequence amino acids 40-89 of Human Macrophage Inflammatory Protein 4 (NP_002979).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 10 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (use citrate buffer pH6.0)
FunctionChemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses.
Tissue specificityExpressed at high levels in lung, lymph nodes, placenta, bone marrow, dendritic cells present in germinal centers and T-cell areas of secondary lymphoid organs and macrophages derived from peripheral blood monocytes. Not expressed by peripheral blood monocytes and a monocyte-to-macrophage differentiation is a prerequisite for expression. Expressed in synovial fluids from patients with rheumatoid and septic arthritis and in ovarian carcinoma ascitic fluid.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling Macrophage Inflammatory Protein 4 with ab104867 at 4-8µg/ml. Arrows inducate positively labelled epithelial cells of intestinal villi. Magnification: 400X.
References for Anti-Macrophage Inflammatory Protein 4 antibody (ab104867)
This product has been referenced in:
Furukawa S et al. Preferential M2 macrophages contribute to fibrosis in IgG4-related dacryoadenitis and sialoadenitis, so-called Mikulicz's disease. Clin Immunol156:9-18 (2015).
Read more (PubMed: 25450336) »
Chen G et al. CC Chemokine Ligand 18 Correlates with Malignant Progression of Prostate Cancer. Biomed Res Int2014:230183 (2014).
Read more (PubMed: 25197632) »