Anti-Macrophage inflammatory protein 5 antibody (ab77004)

Overview

  • Product nameAnti-Macrophage inflammatory protein 5 antibody
    See all Macrophage inflammatory protein 5 primary antibodies
  • Description
    Mouse monoclonal to Macrophage inflammatory protein 5
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: NDAETELMMS KLPLENPVVL NSFHFAADCC TSYISQSIPC SLMKSYFETS SECSKPGVIF LTKKGRQVCA KPSGPGVQDC MKKLKPYSI, corresponding to amino acids 25-113 of Human Macrophage inflammatory protein 5 (NP_116741) with a 26 kDa tag.

  • Positive control
    • Macrophage inflammatory protein 5 transfected 293T cell lysate. Recombinant tagged human Macrophage inflammatory protein 5 fragment.

Properties

Applications

Our Abpromise guarantee covers the use of ab77004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 12 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionChemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15.
  • Tissue specificityMost abundant in heart, skeletal muscle and adrenal gland. Lower levels in placenta, liver, pancreas and bone marrow. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are found in high levels in synovial fluids from rheumatoid patients.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-C motif chemokine 15 antibody
    • CC chemokine 3 antibody
    • CCL15 antibody
    • CCL15(29-92) antibody
    • CCL15_HUMAN antibody
    • Chemokine (C C motif) ligand 15 antibody
    • Chemokine CC-2 antibody
    • Chemokine CC2 antibody
    • HCC-2 antibody
    • HCC2 antibody
    • HMRP 2B antibody
    • HMRP2B antibody
    • Leukotactin 1 antibody
    • Leukotactin-1 antibody
    • LKN-1 antibody
    • LKN1 antibody
    • Macrophage inflammatory protein 5 antibody
    • MIP 1D antibody
    • MIP-1 delta antibody
    • MIP-1D antibody
    • MIP-5 antibody
    • MIP1 delta antibody
    • MIP1d antibody
    • MIP5 antibody
    • MRP 2B antibody
    • Mrp-2b antibody
    • Mrp2b antibody
    • NCC-3 antibody
    • NCC3 antibody
    • New CC chemokine 3 antibody
    • OTTHUMP00000163955 antibody
    • SCYA15 antibody
    • SCYL3 antibody
    • Small inducible cytokine A15 antibody
    • Small inducible cytokine subfamily A (Cys Cys), member 15 antibody
    • Small-inducible cytokine A15 antibody
    • SY15 antibody
    see all

Anti-Macrophage inflammatory protein 5 antibody images

  • Anti-Macrophage inflammatory protein 5 antibody (ab77004) at 5 µg/ml + Recombinant tagged human Macrophage inflammatory protein 5 fragment (the immunogen) at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 12 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Macrophage inflammatory protein 5 antibody (ab77004) at 5 µg/ml

    Lane 1 : Macrophage inflammatory protein 5 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 12 kDa
    Observed band size : 12 kDa

References for Anti-Macrophage inflammatory protein 5 antibody (ab77004)

ab77004 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"