Anti-Macrophage Scavenger Receptor I antibody (ab123946)

Overview

  • Product nameAnti-Macrophage Scavenger Receptor I antibody
    See all Macrophage Scavenger Receptor I primary antibodies
  • Description
    Rabbit polyclonal to Macrophage Scavenger Receptor I
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Cow, Cat, Human, Monkey
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 300-400 of Human Macrophage Scavenger Receptor I (UniProt P21757).

  • Positive control
    • Hela cells; Human liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab123946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ICC/IF 1/50 - 1/200.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionMembrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL). Isoform III does not internalize actetylated LDL.
  • Tissue specificityIsoform I, isoform II and isoform III are expressed in monocyte-derived macrophages.
  • Sequence similaritiesContains 1 collagen-like domain.
    Contains 1 SRCR domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD204 antibody
    • CD204 antigen antibody
    • Macrophage acetylated LDL receptor I and II antibody
    • Macrophage scavenger receptor 1 antibody
    • Macrophage scavenger receptor type III antibody
    • Macrophage scavenger receptor types I and II antibody
    • Msr 1 antibody
    • MSR1 antibody
    • MSRE_HUMAN antibody
    • phSR1 antibody
    • phSR2 antibody
    • SCARA 1 antibody
    • SCARA1 antibody
    • Scavenger receptor class A member 1 antibody
    • Scavenger receptor type A antibody
    • Scvr antibody
    • SR A antibody
    • SRA antibody
    see all

Anti-Macrophage Scavenger Receptor I antibody images

  • Anti-Macrophage Scavenger Receptor I antibody (ab123946) at 0.5 µg/ml + Human liver lysate

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • ab123946, at a 1/50 dilution, staining Macrophage Scavenger Receptor I (green) in Hela cells by Immunofluorescence. Nuclei (blue) are counterstained using Hoechst 33258.

References for Anti-Macrophage Scavenger Receptor I antibody (ab123946)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rhesus monkey Tissue sections (Spleen)
Antigen retrieval step Enzymatic
Permeabilization No
Specification Spleen
Blocking step 2.5% Normal Horse Serum - VectorLabs ImmPRESS™ Excel Amplified HRP Polymer Staining Kit (Anti-Mouse Ig) as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: RT°C
Fixative 10% Neutral Buffered Formalin
Username

Dr. Basel Assaf

Verified customer

Submitted Jun 03 2015

Thank you for kindly confirming these details. I am sorry this vial of antibody has not worked for this customer.

As requested, we are pleased to arrange a free of charge replacement. I can confirm that 1 vial of ab17147 Anti-CD8 antibody [1...

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Thank you for your message and for providing this further information.

I am sorry to hear the resultsare not satiesfactoryon this occasion. I appreciate the time you and your customerhave spent on these experiments, andit is no problem to se...

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Thank you for your enquiry and interest in our products.

The best positive control would be tissue sections from human liver. Bone marrow, spleen, breast tissues may be also suitable but we have not tested these tissue sections with ab123946....

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Thank you for your call today and for your patience while I have been in touch with the lab.

Apparently there have been mixed results with this antibody in mouse samples, and we aren't sure whether it will react with Bl/6 mice. Based on this ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"