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Product name Anti-Mad antibody See all Mad primary antibodies
Rabbit polyclonal to Mad
Tested applications Suitable for:
WB more details
Predicted to work with:
Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
Synthetic peptide (Human)
Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Function Transcriptional repressor. MAD binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MAD thus antagonizes MYC transcriptional activity by competing for MAX.
Sequence similarities Contains 1 basic helix-loop-helix (bHLH) domain.
Cellular localization Nucleus.
Information by UniProt
MAD protein antibody
Anti-Mad antibody images
Western blot - Mad antibody (ab80259)
Anti-Mad antibody (ab80259) at 1 µg/ml + fetal muscle lysate at 10 µg Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution Predicted band size : 25 kDa Observed band size : 30 kDa ( why is the actual band size different from the predicted?) Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Mad antibody (ab80259)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"