• Product nameAnti-MAD1 antibody [9B10]
    See all MAD1 primary antibodies
  • Description
    Mouse monoclonal [9B10] to MAD1
  • Tested applicationsSuitable for: IP, ICC/IF, Flow Cytmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length MAD1 protein (Human)



Our Abpromise guarantee covers the use of ab5783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
ICC/IF 1/500 - 1/1000.
Flow Cyt Use 0.1µg for 106 cells.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionComponent of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.
    • Tissue specificityExpressed weakly at G0/G1 and highly at late S and G2/M phase.
    • Involvement in diseaseDefects in MAD1L1 are involved in the development and/or progression of various types of cancer.
    • Sequence similaritiesBelongs to the MAD1 family.
    • Post-translational
      Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage.
    • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Cytoplasm > cytoskeleton > spindle. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore.
    • Information by UniProt
    • Database links
    • Alternative names
      • hMAD1 antibody
      • HsMAD1 antibody
      • MAD1 antibody
      • MAD1 mitotic arrest deficient like 1 (yeast) antibody
      • MAD1-like protein 1 antibody
      • MAD1L1 antibody
      • MD1L1_HUMAN antibody
      • Mitotic arrest deficient 1 antibody
      • Mitotic arrest deficient 1-like protein 1 antibody
      • Mitotic checkpoint MAD1 protein homolog antibody
      • Mitotic spindle assembly checkpoint protein MAD1 antibody
      • PIG9 antibody
      • Tax binding protein 181 antibody
      • Tax-binding protein 181 antibody
      • TP53I9 antibody
      • Tumor protein p53 inducible protein 9 antibody
      • TXBP181 antibody
      see all

    Anti-MAD1 antibody [9B10] images

    • ab5783 staining MAD1 in a human colon carcinoma cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/100 in 1% PBS BSA) for 1 hour at 37°C. A goat anti-mouse IgG polyclonal was used as the secondary antibody at a dilution of 1/100.

    • Overlay histogram showing HeLa cells stained with ab5783 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab5783, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    References for Anti-MAD1 antibody [9B10] (ab5783)

    This product has been referenced in:
    • Rajanala K  et al. Phosphorylation of nucleoporin Tpr governs its differential localization and is required for its mitotic function. J Cell Sci 127:3505-20 (2014). Read more (PubMed: 24938596) »
    • Hewitt L  et al. Sustained Mps1 activity is required in mitosis to recruit O-Mad2 to the Mad1-C-Mad2 core complex. J Cell Biol 190:25-34 (2010). ICC/IF ; Human . Read more (PubMed: 20624899) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (Colon carcinoma)
    Gel Running Conditions Non-reduced Denaturing (10%)
    Loading amount 40 µg
    Specification Colon carcinoma
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jul 06 2016

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Colon carcinoma)
    Permeabilization Yes - Triton 0.5%
    Specification Colon carcinoma
    Fixative Paraformaldehyde

    Dr. Eleni Petsalaki

    Verified customer

    Submitted Jun 15 2016

    Thank you for your phone call. The originator of ab5783 has informed me that they no longer have the rabbit anti-MAD1 antibody that is mentioned in the image caption. If I can be of further assistance, please let me know.