Overview

  • Product nameAnti-MAD1 antibody
    See all MAD1 primary antibodies
  • Description
    Rabbit polyclonal to MAD1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant fusion protein (S. cerevisiae).

  • Positive control
    • S.cerevisiae whole cell lysate (see images).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: Whole Serum
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Detects a band of approximately 83 kDa.
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionComponent of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.
  • Tissue specificityExpressed weakly at G0/G1 and highly at late S and G2/M phase.
  • Involvement in diseaseDefects in MAD1L1 are involved in the development and/or progression of various types of cancer.
  • Sequence similaritiesBelongs to the MAD1 family.
  • Post-translational
    modifications
    Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage.
  • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Cytoplasm > cytoskeleton > spindle. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore.
  • Information by UniProt
  • Alternative names
    • hMAD1 antibody
    • HsMAD1 antibody
    • MAD1 antibody
    • MAD1 mitotic arrest deficient like 1 (yeast) antibody
    • MAD1-like protein 1 antibody
    • MAD1L1 antibody
    • MD1L1_HUMAN antibody
    • Mitotic arrest deficient 1 antibody
    • Mitotic arrest deficient 1-like protein 1 antibody
    • Mitotic checkpoint MAD1 protein homolog antibody
    • Mitotic spindle assembly checkpoint protein MAD1 antibody
    • PIG9 antibody
    • Tax binding protein 181 antibody
    • Tax-binding protein 181 antibody
    • TP53I9 antibody
    • Tumor protein p53 inducible protein 9 antibody
    • TXBP181 antibody
    see all

Anti-MAD1 antibody images

  • All lanes : Anti-MAD1 antibody (ab11691) at 1/100 dilution

    Lane 1 : Wild type S. cerevisiae whole cell lysate
    Lane 2 : mad1 knockout S. cerevisiae whole cell lysate

    Developed using the ECL technique

    Observed band size : 83 kDa (why is the actual band size different from the predicted?)
  • ab11691 at a 1/100 dilution staining MAD1 in S.cerevisiae by immunofluorescence.

References for Anti-MAD1 antibody (ab11691)

ab11691 has not yet been referenced specifically in any publications.

Product Wall

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