Overview

  • Product name
  • Description
    Rabbit polyclonal to MAD1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-367 of Human MAD1 (Q9Y6D9)

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji cell extracts, paraffin-embedded SW480 Xenograft

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 83 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.
  • Tissue specificity
    Expressed weakly at G0/G1 and highly at late S and G2/M phase.
  • Involvement in disease
    Defects in MAD1L1 are involved in the development and/or progression of various types of cancer.
  • Sequence similarities
    Belongs to the MAD1 family.
  • Post-translational
    modifications
    Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage.
  • Cellular localization
    Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Cytoplasm > cytoskeleton > spindle. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore.
  • Information by UniProt
  • Database links
  • Alternative names
    • hMAD1 antibody
    • HsMAD1 antibody
    • MAD1 antibody
    • MAD1 mitotic arrest deficient like 1 (yeast) antibody
    • MAD1-like protein 1 antibody
    • MAD1L1 antibody
    • MD1L1_HUMAN antibody
    • Mitotic arrest deficient 1 antibody
    • Mitotic arrest deficient 1-like protein 1 antibody
    • Mitotic checkpoint MAD1 protein homolog antibody
    • Mitotic spindle assembly checkpoint protein MAD1 antibody
    • PIG9 antibody
    • Tax binding protein 181 antibody
    • Tax-binding protein 181 antibody
    • TP53I9 antibody
    • Tumor protein p53 inducible protein 9 antibody
    • TXBP181 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded SW480 Xenograft labeling MAD1 using ab155236 at 1/100 dilution.
  • Anti-MAD1 antibody (ab155236) at 1/10000 dilution + Molt-4 whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 83 kDa

References

ab155236 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab155236.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up