Overview

  • Product name
  • Description
    Rabbit polyclonal to MAD1
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region between residues 1 and 50 of human MAD1 using the numbering given in Swiss-Prot entry Q9Y6D9.

  • Positive control
    • Human prostate adenocarcinoma tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antibody was affinity purified using an epitope specific to MAD1 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500.

    Epitope exposure is recommended.
    Epitope exposure with citrate buffer will enhance staining.
    Likely to work with frozen sections.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.
    • Tissue specificity
      Expressed weakly at G0/G1 and highly at late S and G2/M phase.
    • Involvement in disease
      Defects in MAD1L1 are involved in the development and/or progression of various types of cancer.
    • Sequence similarities
      Belongs to the MAD1 family.
    • Post-translational
      modifications
      Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage.
    • Cellular localization
      Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Cytoplasm > cytoskeleton > spindle. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore.
    • Information by UniProt
    • Database links
    • Alternative names
      • hMAD1 antibody
      • HsMAD1 antibody
      • MAD1 antibody
      • MAD1 mitotic arrest deficient like 1 (yeast) antibody
      • MAD1-like protein 1 antibody
      • MAD1L1 antibody
      • MD1L1_HUMAN antibody
      • Mitotic arrest deficient 1 antibody
      • Mitotic arrest deficient 1-like protein 1 antibody
      • Mitotic checkpoint MAD1 protein homolog antibody
      • Mitotic spindle assembly checkpoint protein MAD1 antibody
      • PIG9 antibody
      • Tax binding protein 181 antibody
      • Tax-binding protein 181 antibody
      • TP53I9 antibody
      • Tumor protein p53 inducible protein 9 antibody
      • TXBP181 antibody
      see all

    Images

    • IHC analysis of FFPE section of human prostate adenocarcinoma using ab84490 at a dilution of 1/250.

    References

    ab84490 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab84490.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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