Recombinant Anti-MAD1L1/MAD1 antibody [EPR14676] (ab184175)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14676] to MAD1L1/MAD1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MAD1L1/MAD1 antibody [EPR14676]
See all MAD1L1/MAD1 primary antibodies -
Description
Rabbit monoclonal [EPR14676] to MAD1L1/MAD1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2, MCF7, HeLa and A431 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR14676 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). |
Target
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Function
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding. -
Tissue specificity
Expressed weakly at G0/G1 and highly at late S and G2/M phase. -
Involvement in disease
Defects in MAD1L1 are involved in the development and/or progression of various types of cancer. -
Sequence similarities
Belongs to the MAD1 family. -
Post-translational
modificationsPhosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage. -
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Cytoplasm > cytoskeleton > spindle. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore. - Information by UniProt
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Database links
- Entrez Gene: 8379 Human
- Entrez Gene: 17120 Mouse
- Entrez Gene: 680006 Rat
- Omim: 602686 Human
- SwissProt: Q9Y6D9 Human
- SwissProt: Q9WTX8 Mouse
- Unigene: 654838 Human
- Unigene: 27250 Mouse
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Alternative names
- hMAD1 antibody
- HsMAD1 antibody
- MAD1 antibody
see all
Images
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All lanes : Anti-MAD1L1/MAD1 antibody [EPR14676] (ab184175) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 83 kDa -
All lanes : Anti-MAD1L1/MAD1 antibody [EPR14676] (ab184175) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 83 kDa
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab184175 has not yet been referenced specifically in any publications.