Anti-MAD1 antibody [MAD1D6H4] (ab50791)

Overview

  • Product nameAnti-MAD1 antibody [MAD1D6H4]
    See all MAD1 primary antibodies
  • Description
    Mouse monoclonal [MAD1D6H4] to MAD1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human MAD1

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, 8mM Sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThe antibody was filtered through an 0.22 µm membrane.
  • ClonalityMonoclonal
  • Clone numberMAD1D6H4
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 83 kDa.

Target

  • FunctionComponent of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.
  • Tissue specificityExpressed weakly at G0/G1 and highly at late S and G2/M phase.
  • Involvement in diseaseDefects in MAD1L1 are involved in the development and/or progression of various types of cancer.
  • Sequence similaritiesBelongs to the MAD1 family.
  • Post-translational
    modifications
    Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage.
  • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Cytoplasm > cytoskeleton > spindle. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore.
  • Information by UniProt
  • Database links
  • Alternative names
    • hMAD1 antibody
    • HsMAD1 antibody
    • MAD1 antibody
    • MAD1 mitotic arrest deficient like 1 (yeast) antibody
    • MAD1-like protein 1 antibody
    • MAD1L1 antibody
    • MD1L1_HUMAN antibody
    • Mitotic arrest deficient 1 antibody
    • Mitotic arrest deficient 1-like protein 1 antibody
    • Mitotic checkpoint MAD1 protein homolog antibody
    • Mitotic spindle assembly checkpoint protein MAD1 antibody
    • PIG9 antibody
    • Tax binding protein 181 antibody
    • Tax-binding protein 181 antibody
    • TP53I9 antibody
    • Tumor protein p53 inducible protein 9 antibody
    • TXBP181 antibody
    see all

Anti-MAD1 antibody [MAD1D6H4] images

References for Anti-MAD1 antibody [MAD1D6H4] (ab50791)

ab50791 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Permeabilization No
Username

Dr. Kirk McManus

Verified customer

Submitted May 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"