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Recombinant human Mad2.
Our Abpromise guarantee covers the use of ab10691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 24.6 kDa).|
|IP||Use a concentration of 5 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
ab10691 staining Mad2L1 in colon cancer tissue, after antigen retreival, by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Western blot using ab10691 at 1/500.
Lane 1: Hela Nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: Jurkat
Lane 5: 293
Mad2L1 predicted size: 24.6kD.
Secondary ab: Rabbit polyclonal to Mouse IgG H&L (HRP) ab6728 (1/5000)
Exposure time: 1min
20µg of lysate per lane.
Western blot using ab10691 at 1/500. Lane 1: Hela Nuclear Lane 2: Hela Lane 3: A431 Lane 4: Jurkat Lane 5: 293 Mad2L1 predicted size: 24.6kD. Secondary ab: anti-mouse HRP (ab6728) (1/5000) Exposure time: 1min 20µg of lysate per lane.
This image is courtesy of an Abreview submitted by Saira SarfrazMad2L1 antibody (ab10691) at 1/1000 (in TBS for 90 minutes at 22°C) + whole tissue lysate of Human liver biopsy (chronic hepatitis C - (HepC) or non-viral (NVH)) patients at 50µg.
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