Anti-Mad2L1 antibody [OTI4D2] (ab114036)
Key features and details
- Mouse monoclonal [OTI4D2] to Mad2L1
- Suitable for: WB, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG2a
Overview
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Product name
Anti-Mad2L1 antibody [OTI4D2]
See all Mad2L1 primary antibodies -
Description
Mouse monoclonal [OTI4D2] to Mad2L1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant full length protein corresponding to Human Mad2L1 aa 1 to the C-terminus. Produced in HEK-293T cells (NP_002349).
Database link: Q13257 -
Positive control
- WB: HEK-293T cell lysate transfected with or pCMV6-ENTRY Mad2L1 cDNA. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY Mad2L1.
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General notes
Clone OTI4D2 (formerly 4D2).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI4D2 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab114036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 24 kDa.
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ICC/IF | (1) |
1/100.
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Notes |
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WB
1/1000. Predicted molecular weight: 24 kDa. |
ICC/IF
1/100. |
Target
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Function
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. -
Sequence similarities
Belongs to the MAD2 family.
Contains 1 HORMA domain. -
Domain
The protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. The protein in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and of MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed form, and thereby promotes interaction with CDC20. -
Post-translational
modificationsPhosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20. -
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Cytoplasm. Recruited by MAD1L1 to unattached kinetochores (Probable). Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD. - Information by UniProt
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Database links
- Entrez Gene: 4085 Human
- Entrez Gene: 56150 Mouse
- Entrez Gene: 297176 Rat
- Omim: 601467 Human
- SwissProt: Q13257 Human
- SwissProt: Q9Z1B5 Mouse
- Unigene: 591697 Human
- Unigene: 485053 Mouse
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Alternative names
- HsMAD 2 antibody
- HsMAD2 antibody
- MAD 2 antibody
see all
Images
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All lanes : Anti-Mad2L1 antibody [OTI4D2] (ab114036) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with the pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with the pCMV6-ENTRY Mad2L1 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 24 kDa -
COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY Mad2L1 stained for Mad2L1 (green) using ab114036 at 1/100 dilution in ICC/IF.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab114036 has not yet been referenced specifically in any publications.