Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab24588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 24 kDa.
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionComponent of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
  • Sequence similaritiesBelongs to the MAD2 family.
    Contains 1 HORMA domain.
  • DomainThe protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. The protein in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and of MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed form, and thereby promotes interaction with CDC20.
  • Post-translational
    modifications
    Phosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20.
  • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Cytoplasm. Recruited by MAD1L1 to unattached kinetochores (Probable). Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homolog of MAD2 S cerevisiae antibody
    • HsMAD 2 antibody
    • HsMAD2 antibody
    • Human mitotic feedback control protein Madp2 antibody
    • MAD 2 antibody
    • MAD 2 like 1 antibody
    • MAD 2 like protein 1 antibody
    • MAD 2 mitotic arrest deficient like 1 antibody
    • MAD 2A antibody
    • MAD2 antibody
    • MAD2 L1 antibody
    • MAD2 like 1 antibody
    • MAD2 like protein 1 antibody
    • MAD2 mitotic arrest deficient like 1 antibody
    • MAD2-like protein 1 antibody
    • MAD2A antibody
    • Mad2l1 antibody
    • MD2L1_HUMAN antibody
    • Mitotic arrest deficient 2-like protein 1 antibody
    • Mitotic arrest deficient like 1 antibody
    • Mitotic arrest deficient yeast homolog antibody
    • Mitotic arrest deficient yeast homolog like 1 antibody
    • Mitotic spindle assembly checkpoint protein antibody
    • Mitotic spindle assembly checkpoint protein MAD2A antibody
    see all

Anti-Mad2L1 antibody images

  • ab24588 at a 1/100 dilution staining human breast cancer cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 5% BSA prior to incubation with the antibody for 1 hour. A TRITC conjugated polyclonal anti-rabbit Ig was used as the secondary antibody. The image shows staining with ab24588 as well as a DNA stain. The right hand panel shows a merged image.

    See Abreview

References for Anti-Mad2L1 antibody (ab24588)

This product has been referenced in:
  • Ingemarsdotter CK  et al. Low-dose paclitaxel synergizes with oncolytic adenoviruses via mitotic slippage and apoptosis in ovarian cancer. Oncogene 29:6051-63 (2010). WB . Read more (PubMed: 20729921) »
  • Gisselsson D  et al. When the genome plays dice: circumvention of the spindle assembly checkpoint and near-random chromosome segregation in multipolar cancer cell mitoses. PLoS ONE 3:e1871 (2008). ICC/IF ; Human . Read more (PubMed: 18392149) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (breast cancer)
Specification breast cancer
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 5 minute(s) · Concentration: 5%
Username

Dr. Lee Bergman

Verified customer

Submitted Sep 08 2006

I am very happy to hear the replacement vial worked for you and look forward to reading the Abreview. Please do not hesitate to contact me if I can be of further help. I wish you all the best with your research,

Thank you for your enquiry. In general we recommend that users apply the ABC technique as this amplifies the signal significantly. However, on this occasion the antibody has been optimised using fluorescence detection and therefore I would like to ...

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I have just received feedback from the source of ab24588 and can confirm that the laboratory would expect the antibody to work in one of the many conditions you have tested. I suspect the antibody was damaged during storage or shipping and can confirm ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"