Anti-Mad2L1 antibody (ab97777)


  • Product nameAnti-Mad2L1 antibody
    See all Mad2L1 primary antibodies
  • Description
    Rabbit polyclonal to Mad2L1
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 192 and 205 of Human Mad2L1 (NP_002349).

  • Positive control
    • NCIN87, U2OS, HepG2, MOLT4 and Raji cell lines; DLD-1 xenograft



Our Abpromise guarantee covers the use of ab97777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 24 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.


  • FunctionComponent of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
  • Sequence similaritiesBelongs to the MAD2 family.
    Contains 1 HORMA domain.
  • DomainThe protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. The protein in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and of MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed form, and thereby promotes interaction with CDC20.
  • Post-translational
    Phosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20.
  • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Cytoplasm. Recruited by MAD1L1 to unattached kinetochores (Probable). Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD.
  • Information by UniProt
  • Database links
  • Alternative names
    • HsMAD 2 antibody
    • HsMAD2 antibody
    • MAD 2 antibody
    • MAD 2A antibody
    • MAD2 like 1 antibody
    • MAD2 like protein 1 antibody
    • MAD2 mitotic arrest deficient like 1 antibody
    • MAD2-like protein 1 antibody
    • Mad2l1 antibody
    • MD2L1_HUMAN antibody
    • Mitotic arrest deficient 2-like protein 1 antibody
    • Mitotic arrest deficient yeast homolog like 1 antibody
    • Mitotic spindle assembly checkpoint protein MAD2A antibody
    see all

Anti-Mad2L1 antibody images

  • All lanes : Anti-Mad2L1 antibody (ab97777) at 1/1000 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : MOLT4 whole cell lysate
    Lane 3 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 24 kDa
  • Immunofluorescence analysis of Mad2L1 in Human osteosarcoma cell line U2OS, using ab97777 at 1/1000 dilution.
    1: Mad2L1
    2: ACA
    3: merged with DAPI
    Cells were fixed with 4% PFA/PBS for 15min, RT and blocked with 2.5% BSA/PBS.
  • Immunohistochemical analysis of Mad2L1 in paraffin embedded DLD-1 xenograft, using ab97777 at a 1/500 dilution.

References for Anti-Mad2L1 antibody (ab97777)

ab97777 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (HCV infected patients liver)
Specification HCV infected patients liver
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Saira Khalid

Verified customer

Submitted Dec 17 2012

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