Anti-MAD2L1 binding protein antibody (ab37584)

Overview

  • Product nameAnti-MAD2L1 binding protein antibody
    See all MAD2L1 binding protein primary antibodies
  • Description
    Chicken polyclonal to MAD2L1 binding protein
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity

    Predicted to work with: Human
  • Immunogen

    Synthetic peptide, corresponding to N terminal amino acids 1-126 of MAD2L1 binding protein

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/2000. Predicted molecular weight: 31 kDa. We recommend using about 2.5 ug of total protein per lane in the colorimetric detection method, and about 0.25µg for ECL detection.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex.
    • Sequence similaritiesBelongs to the MAD2L1BP family.
    • Developmental stageDuring the cell cycle, levels increase and then remain constant until late mitosis after which they drop.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus. Cytoplasm > cytoskeleton > spindle. During early mitosis, unevenly distributed throughout the nucleoplasm. From metaphase to anaphase, concentrated on the spindle.
    • Information by UniProt
    • Database links
    • Alternative names
      • Caught by MAD2 protein antibody
      • CMT2 antibody
      • dJ261G23.1 antibody
      • KIAA0110 antibody
      • MAD2L1 binding protein antibody
      • MAD2L1 binding protein isoform 2 antibody
      • MAD2L1-binding protein antibody
      • Mad2l1bp antibody
      • MD2BP_HUMAN antibody
      • MGC11282 antibody
      • OTTHUMP00000016496 antibody
      • RP1 261G23.6 antibody
      see all

    Anti-MAD2L1 binding protein antibody images

    • Anti-MAD2L1 binding protein antibody (ab37584) at 1/2000 dilution + E coli-derived fusion protein at 2.5 µg

      Secondary
      Goat anti-IgY-HRP at 1/1000 dilution

      Predicted band size : 31 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)

    References for Anti-MAD2L1 binding protein antibody (ab37584)

    ab37584 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab37584.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"