Anti-MAD2L1 binding protein antibody (ab37584)

Overview

  • Product name
    Anti-MAD2L1 binding protein antibody
    See all MAD2L1 binding protein primary antibodies
  • Description
    Chicken polyclonal to MAD2L1 binding protein
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity

    Predicted to work with: Human
  • Immunogen

    Synthetic peptide, corresponding to N terminal amino acids 1-126 of MAD2L1 binding protein

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/2000. Predicted molecular weight: 31 kDa. We recommend using about 2.5 ug of total protein per lane in the colorimetric detection method, and about 0.25µg for ECL detection.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex.
    • Sequence similarities
      Belongs to the MAD2L1BP family.
    • Developmental stage
      During the cell cycle, levels increase and then remain constant until late mitosis after which they drop.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus. Cytoplasm > cytoskeleton > spindle. During early mitosis, unevenly distributed throughout the nucleoplasm. From metaphase to anaphase, concentrated on the spindle.
    • Information by UniProt
    • Database links
    • Alternative names
      • Caught by MAD2 protein antibody
      • CMT2 antibody
      • dJ261G23.1 antibody
      • KIAA0110 antibody
      • MAD2L1 binding protein antibody
      • MAD2L1 binding protein isoform 2 antibody
      • MAD2L1-binding protein antibody
      • Mad2l1bp antibody
      • MD2BP_HUMAN antibody
      • MGC11282 antibody
      • OTTHUMP00000016496 antibody
      • RP1 261G23.6 antibody
      see all

    Images

    • Anti-MAD2L1 binding protein antibody (ab37584) at 1/2000 dilution + E coli-derived fusion protein at 2.5 µg

      Secondary
      Goat anti-IgY-HRP at 1/1000 dilution

      Predicted band size : 31 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)

    References

    ab37584 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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