Anti-MAD2L1 binding protein antibody (ab77206)


  • Product nameAnti-MAD2L1 binding protein antibody
    See all MAD2L1 binding protein primary antibodies
  • Description
    Mouse monoclonal to MAD2L1 binding protein
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1 - 275 of Human MAD2L1 binding protein (AAH02904), with a 26 kDa tag

  • Positive control
    • HeLa nuclear extract; immunogen


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab77206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 31 kDa.
ELISA Use at an assay dependent dilution.


  • FunctionMay function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex.
  • Sequence similaritiesBelongs to the MAD2L1BP family.
  • Developmental stageDuring the cell cycle, levels increase and then remain constant until late mitosis after which they drop.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Cytoplasm > cytoskeleton > spindle. During early mitosis, unevenly distributed throughout the nucleoplasm. From metaphase to anaphase, concentrated on the spindle.
  • Information by UniProt
  • Database links
  • Alternative names
    • Caught by MAD2 protein antibody
    • CMT2 antibody
    • dJ261G23.1 antibody
    • KIAA0110 antibody
    • MAD2L1 binding protein antibody
    • MAD2L1 binding protein isoform 2 antibody
    • MAD2L1-binding protein antibody
    • Mad2l1bp antibody
    • MD2BP_HUMAN antibody
    • MGC11282 antibody
    • OTTHUMP00000016496 antibody
    • RP1 261G23.6 antibody
    see all

Anti-MAD2L1 binding protein antibody images

  • Anti-MAD2L1 binding protein antibody (ab77206) at 1/500 dilution + recombinant tagged MAD2L1 protein (the immunogen) at 0.2 µg

    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 31 kDa
    Observed band size : 56 kDa (why is the actual band size different from the predicted?)
  • Anti-MAD2L1 binding protein antibody (ab77206) at 1/500 dilution + HeLa nuclear extract cell lysate at 25 µg

    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 31 kDa
    Observed band size : 31 kDa

References for Anti-MAD2L1 binding protein antibody (ab77206)

ab77206 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.
I am not sure why the current vial of the antibody that you received was not as efficient as before. I am going to send you a new vial free of charge and hopefully that one should not have the same issues. Your new order ...

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Thank you for contacting Abcam.
We have not heard of any other similar issue regarding this product and it is a mouse monoclonal, so there should not be much, if any batch to batch variation. Also the storage and buffer conditions have not changed...

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