Anti-MAD2L1 binding protein antibody (ab88611)

Overview

  • Product nameAnti-MAD2L1 binding protein antibody
    See all MAD2L1 binding protein primary antibodies
  • Description
    Mouse polyclonal to MAD2L1 binding protein
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human MAD2L1 binding protein (AAH02904).

  • Positive control
    • WB: A-431 cell lysate and MAD2L1 binding protein transfected 293T cell lysate ICC/IF: HeLa cell

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab88611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionMay function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex.
  • Sequence similaritiesBelongs to the MAD2L1BP family.
  • Developmental stageDuring the cell cycle, levels increase and then remain constant until late mitosis after which they drop.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Cytoplasm > cytoskeleton > spindle. During early mitosis, unevenly distributed throughout the nucleoplasm. From metaphase to anaphase, concentrated on the spindle.
  • Information by UniProt
  • Database links
  • Alternative names
    • Caught by MAD2 protein antibody
    • CMT2 antibody
    • dJ261G23.1 antibody
    • KIAA0110 antibody
    • MAD2L1 binding protein antibody
    • MAD2L1 binding protein isoform 2 antibody
    • MAD2L1-binding protein antibody
    • Mad2l1bp antibody
    • MD2BP_HUMAN antibody
    • MGC11282 antibody
    • OTTHUMP00000016496 antibody
    • RP1 261G23.6 antibody
    see all

Anti-MAD2L1 binding protein antibody images

  • Anti-MAD2L1 binding protein antibody (ab88611) at 1 µg/ml + A-431 cell lysate at 50 µg

    Predicted band size : 31 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-MAD2L1 binding protein antibody (ab88611) at 1 µg/ml

    Lane 1 : MAD2L1 binding protein transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 31 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
  • ab88611, at 10 µg/ml, staining MAD2L1 binding protein in HeLa cell by Immunofluorescence.

References for Anti-MAD2L1 binding protein antibody (ab88611)

ab88611 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"