Recombinant
RabMAb

Anti-MAD2L1 binding protein antibody [EPR9584] (ab150363)

Overview

  • Product name
    Anti-MAD2L1 binding protein antibody [EPR9584]
    See all MAD2L1 binding protein primary antibodies
  • Description
    Rabbit monoclonal [EPR9584] to MAD2L1 binding protein
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • HepG2, A431, and HeLa cell lysates, Human brain and Human cervical carcinoma tissues This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR9584
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      May function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex.
    • Sequence similarities
      Belongs to the MAD2L1BP family.
    • Developmental stage
      During the cell cycle, levels increase and then remain constant until late mitosis after which they drop.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus. Cytoplasm > cytoskeleton > spindle. During early mitosis, unevenly distributed throughout the nucleoplasm. From metaphase to anaphase, concentrated on the spindle.
    • Information by UniProt
    • Database links
    • Alternative names
      • Caught by MAD2 protein antibody
      • CMT2 antibody
      • dJ261G23.1 antibody
      • KIAA0110 antibody
      • MAD2L1 binding protein antibody
      • MAD2L1 binding protein isoform 2 antibody
      • MAD2L1-binding protein antibody
      • Mad2l1bp antibody
      • MD2BP_HUMAN antibody
      • MGC11282 antibody
      • OTTHUMP00000016496 antibody
      • RP1 261G23.6 antibody
      see all

    Images

    • ab150363 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab150363 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • All lanes : Anti-MAD2L1 binding protein antibody [EPR9584] (ab150363) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 31 kDa
      Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin embedded Human brain tissue labelling MAD2L1 binding protein with ab150363 antibody at a dilution of 1/100.
    • Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labelling MAD2L1 binding protein with ab150363 antibody at a dilution of 1/100.

    References

    This product has been referenced in:
    • Shin HJ  et al. p31comet-Induced Cell Death Is Mediated by Binding and Inactivation of Mad2. PLoS One 10:e0141523 (2015). WB . Read more (PubMed: 26544187) »
    • de Lange J  et al. Defective sister chromatid cohesion is synthetically lethal with impaired APC/C function. Nat Commun 6:8399 (2015). WB . Read more (PubMed: 26423134) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab150363.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up