• Product nameAnti-Mad2L2 antibody
    See all Mad2L2 primary antibodies
  • Description
    Rabbit polyclonal to Mad2L2
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Pig, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide:


    conjugated to KLH, corresponding to amino acids 3-14 of Human Mad2L2 (NP_006332.2).

  • Positive control
    • Human Brain, Cerebellum tissue.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 0.27% Potassium phosphate
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab115622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 24 kDa.Can be blocked with Human Mad2L2 peptide (ab11254).
ELISA 1/7500 - 1/30000.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.


  • FunctionAdapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesContains 1 HORMA domain.
  • Cellular localizationNucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homolog of REV7 S cerevisiae antibody
    • hREV7 antibody
    • MAD2 (mitotic arrest deficient yeast, homolog) like 2 antibody
    • MAD2 homolog antibody
    • MAD2 like 2 antibody
    • MAD2 mitotic arrest deficient like 2 antibody
    • MAD2-like protein 2 antibody
    • MAD2B antibody
    • Mad2l2 antibody
    • MD2L2_HUMAN antibody
    • Mitotic Arrest Deficient 2 L2 antibody
    • Mitotic arrest deficient 2-like protein 2 antibody
    • Mitotic arrest deficient homolog like 2 antibody
    • Mitotic arrest deficient like 2 (yeast) antibody
    • Mitotic arrest deficient yeast homolog antibody
    • Mitotic spindle assembly checkpoint protein MAD2B antibody
    • Polymerase (DNA directed) zeta 2 accessory subunit antibody
    • POLZ2 antibody
    • REV 7 antibody
    • REV7 antibody
    • REV7 homolog antibody
    • Weakly similar to Mitotic MAD2 protein (S cerevisiae) antibody
    see all

Anti-Mad2L2 antibody images

  • All lanes : Anti-Mad2L2 antibody (ab115622) at 1/1000 dilution

    Lane 1 : Mouse E10.5 whole embryo lysate
    Lane 2 : Mouse E12.5 whole embryo lysate
    Lane 3 : Mouse E17.5 whole brain lysate
    Lane 4 : HeLa cell lysate

    HRP-conjugated Goat anti-rabbit IgG at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 24 kDa

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab115622 staining Mad2L2 (green) in Human lung cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with the Hope technique. Samples were incubated with primary antibody (1/300) for 30 minutes at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Right - negative control.

    See Abreview

  • ab115622 staining Mad2L2 in Human lung cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with the Hope Technique and blocked for 15 minutes at 25°C. Samples were incubated with primary antibody (1/300) for 1 hour at 25°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab115622, at 10µg/ml, staining Mad2L2 in Formalin-fixed, Paraffin-embedded Human Brain Cerebellum tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-Mad2L2 antibody (ab115622)

ab115622 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 0 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Mouse Tissue lysate - whole (E12.5 whole embryo lysate)
Specification E12.5 whole embryo lysate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (lung cancer tissue)
Specification lung cancer tissue
Permeabilization No
Fixative Hope-Technique

Abcam user community

Verified customer

Submitted Jul 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Zytomed Systems HRP Polymer Kit Blocking Solution as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 25°C
Antigen retrieval step None
Sample Human Tissue sections (Lung cancer)
Specification Lung cancer
Permeabilization No
Fixative Hope-Technique

Abcam user community

Verified customer

Submitted Jun 20 2013