• Product nameAnti-Mad2L2 antibody
    See all Mad2L2 primary antibodies
  • Description
    Rabbit polyclonal to Mad2L2
  • SpecificityThis antibody detects a single band just below the 21kD marker, that corresponds to Mad2L2 / Mad2B.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Xenopus laevis
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 3-14 of Human Mad2L2.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab3630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesWB: 1/500. Detects a band of approximately ~20 kDa (predicted molecular weight: 24 kDa). Can be blocked with Mad2L2 (aa 3-14) peptide (human)

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAdapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation.
    • Tissue specificityUbiquitously expressed.
    • Sequence similaritiesContains 1 HORMA domain.
    • Cellular localizationNucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm.
    • Information by UniProt
    • Database links
      • Alternative names
        • Homolog of REV7 S cerevisiae antibody
        • hREV7 antibody
        • MAD2 (mitotic arrest deficient yeast, homolog) like 2 antibody
        • MAD2 homolog antibody
        • MAD2 like 2 antibody
        • MAD2 mitotic arrest deficient like 2 antibody
        • MAD2-like protein 2 antibody
        • MAD2B antibody
        • Mad2l2 antibody
        • MD2L2_HUMAN antibody
        • Mitotic Arrest Deficient 2 L2 antibody
        • Mitotic arrest deficient 2-like protein 2 antibody
        • Mitotic arrest deficient homolog like 2 antibody
        • Mitotic arrest deficient like 2 (yeast) antibody
        • Mitotic arrest deficient yeast homolog antibody
        • Mitotic spindle assembly checkpoint protein MAD2B antibody
        • Polymerase (DNA directed) zeta 2 accessory subunit antibody
        • POLZ2 antibody
        • REV 7 antibody
        • REV7 antibody
        • REV7 homolog antibody
        • Weakly similar to Mitotic MAD2 protein (S cerevisiae) antibody
        see all

      Anti-Mad2L2 antibody images

      • All lanes : Anti-Mad2L2 antibody (ab3630) at 1/500 dilution

        Lane 1 : Whole cell extract prepared from Hela cells
        Lane 2 : Nuclear lysates prepared from Hela cells

        Lysates/proteins at 20 µg per lane.

        Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

        Predicted band size : 24 kDa

      References for Anti-Mad2L2 antibody (ab3630)

      ab3630 has not yet been referenced specifically in any publications.

      Product Wall

      Abcam has not validated the combination of species/application used in this Abreview.
      Application Western blot
      Sample Zebrafish Tissue lysate - whole (whole larvae)
      Loading amount 100 µg
      Specification whole larvae
      Gel Running Conditions Reduced Denaturing
      Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

      Mr. Gregory Weber

      Verified customer

      Submitted Aug 04 2011