Overview

  • Product name
  • Description
    Rabbit polyclonal to Mad2L2
  • Specificity
    This antibody detects a single band just below the 21kD marker, that corresponds to Mad2L2 / Mad2B.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Xenopus laevis
  • Immunogen

    Synthetic peptide:

    TLTRQDLNFGQV

    , corresponding to amino acids 3-14 of Human Mad2L2.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/500. Detects a band of approximately ~20 kDa (predicted molecular weight: 24 kDa). Can be blocked with Mad2L2 (aa 3-14) peptide (human)

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation.
    • Tissue specificity
      Ubiquitously expressed.
    • Sequence similarities
      Contains 1 HORMA domain.
    • Cellular localization
      Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm.
    • Information by UniProt
    • Database links
      • Alternative names
        • Homolog of REV7 S cerevisiae antibody
        • hREV7 antibody
        • MAD2 (mitotic arrest deficient yeast, homolog) like 2 antibody
        • MAD2 homolog antibody
        • MAD2 like 2 antibody
        • MAD2 mitotic arrest deficient like 2 antibody
        • MAD2-like protein 2 antibody
        • MAD2B antibody
        • Mad2l2 antibody
        • MD2L2_HUMAN antibody
        • Mitotic Arrest Deficient 2 L2 antibody
        • Mitotic arrest deficient 2-like protein 2 antibody
        • Mitotic arrest deficient homolog like 2 antibody
        • Mitotic arrest deficient like 2 (yeast) antibody
        • Mitotic arrest deficient yeast homolog antibody
        • Mitotic spindle assembly checkpoint protein MAD2B antibody
        • Polymerase (DNA directed) zeta 2 accessory subunit antibody
        • POLZ2 antibody
        • REV 7 antibody
        • REV7 antibody
        • REV7 homolog antibody
        • Weakly similar to Mitotic MAD2 protein (S cerevisiae) antibody
        see all

      Anti-Mad2L2 antibody images

      • All lanes : Anti-Mad2L2 antibody (ab3630) at 1/500 dilution

        Lane 1 : Whole cell extract prepared from Hela cells
        Lane 2 : Nuclear lysates prepared from Hela cells

        Lysates/proteins at 20 µg per lane.

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

        Predicted band size : 24 kDa

      References for Anti-Mad2L2 antibody (ab3630)

      ab3630 has not yet been referenced specifically in any publications.

      Product Wall

      Abreviews
      Abcam has not validated the combination of species/application used in this Abreview.
      Application
      Western blot
      Sample
      Zebrafish Tissue lysate - whole (whole larvae)
      Loading amount
      100 µg
      Specification
      whole larvae
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Username

      Mr. Gregory Weber

      Verified customer

      Submitted Aug 04 2011

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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