The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/1000. Predicted molecular weight: 24 kDa.
Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation.
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Mad2L2 with ab129199 at 1/500. Cells were fixed with 4% Paraformaldehyde. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Immunoprecipitation of Hela cells labeling Mad2L2 using ab180579 at 1/40 dilution (lane 1) or negative control (lane 2) followed by anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.
Immunohistochemical staining of paraffin-embedded Human ovarian carcinoma labeling Mad2L2 at using ab180579 1/50 dilution and ImmunoHistoprobe HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.
Western blot - Anti-Mad2L2 [EPR13657] antibody (ab180579)
All lanes : Anti-Mad2L2 antibody [EPR13657] (ab180579) at 1/1000 dilution
Lane 1 : K562 cell lysate Lane 2 : SW480 cell lysate Lane 3 : Hela cell lysate Lane 4 : Molt-4 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution