Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab3995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.

Target

  • Function
    Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHc13 antibody
    • Class C basic helix-loop-helix protein 13 antibody
    • FLJ35523 antibody
    • Likely ortholog of mouse Max dimerization protein 3 antibody
    • MAD3_HUMAN antibody
    • MAX dimerization protein 3 antibody
    • Max dimerizer 3 antibody
    • Max-associated protein 3 antibody
    • Max-interacting transcriptional repressor MAD3 antibody
    • MGC2383 antibody
    • mxd3 antibody
    • Myx antibody
    • OTTHUMP00000223596 antibody
    • OTTHUMP00000223598 antibody
    see all

Images

  • ab3995 (1/200) staining MAD3 in HeLa cells (green). Cells were fixed in methanol, permeabilised in 0.5% Triton X-100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

    See Abreview

References

ab3995 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
Yes - 0.5% Triton X100 in PBS
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Oct 30 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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