Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab3995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.

Target

  • FunctionTranscriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHc13 antibody
    • Class C basic helix-loop-helix protein 13 antibody
    • FLJ35523 antibody
    • Likely ortholog of mouse Max dimerization protein 3 antibody
    • MAD3_HUMAN antibody
    • MAX dimerization protein 3 antibody
    • Max dimerizer 3 antibody
    • Max-associated protein 3 antibody
    • Max-interacting transcriptional repressor MAD3 antibody
    • MGC2383 antibody
    • mxd3 antibody
    • Myx antibody
    • OTTHUMP00000223596 antibody
    • OTTHUMP00000223598 antibody
    see all

Anti-MAD3 antibody images

  • ab3995 (1/200) staining MAD3 in HeLa cells (green). Cells were fixed in methanol, permeabilised in 0.5% Triton X-100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

    See Abreview

References for Anti-MAD3 antibody (ab3995)

ab3995 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Permeabilization Yes - 0.5% Triton X100 in PBS
Username

Dr. Kirk McManus

Verified customer

Submitted Oct 30 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"