Overview

  • Product nameAnti-MAD3 antibody [EPR3882]
    See all MAD3 primary antibodies
  • Description
    Rabbit monoclonal [EPR3882] to MAD3
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human MAD3.

  • Positive control
    • HeLa and C6 cell lysates. Human brain, breast and testis tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab108525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IHC-P 1/100 - 1/250. For antigen retrieval, heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC or IP.
  • Target

    • FunctionTranscriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.
    • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • bHLHc13 antibody
      • Class C basic helix-loop-helix protein 13 antibody
      • FLJ35523 antibody
      • Likely ortholog of mouse Max dimerization protein 3 antibody
      • MAD3_HUMAN antibody
      • MAX dimerization protein 3 antibody
      • Max dimerizer 3 antibody
      • Max-associated protein 3 antibody
      • Max-interacting transcriptional repressor MAD3 antibody
      • MGC2383 antibody
      • mxd3 antibody
      • Myx antibody
      • OTTHUMP00000223596 antibody
      • OTTHUMP00000223598 antibody
      see all

    Anti-MAD3 antibody [EPR3882] images

    • All lanes : Anti-MAD3 antibody [EPR3882] (ab108525) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : C6 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 23 kDa
    • ab108525 at 1/100 dilution staining MAD3 in paraffin-embedded Human brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    • ab108525 at 1/100 dilution staining MAD3 in paraffin-embedded Human breast tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    • ab108525 at 1/100 dilution staining MAD3 in paraffin-embedded Human testis tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    References for Anti-MAD3 antibody [EPR3882] (ab108525)

    ab108525 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab108525.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"