Overview

  • Product name
    Anti-MAD4 antibody
    See all MAD4 primary antibodies
  • Description
    Goat polyclonal to MAD4
  • Host species
    Goat
  • Specificity
    The appearance of the 22 kDa band was blocked by incubation with the immunising peptide.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    GPHCRRLGRPALS

    , corresponding to C terminal amino acids 197-209 of Human MAD4.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

Applications

Our Abpromise guarantee covers the use of ab15841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 23 kDa).

Target

  • Function
    Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.
  • Sequence similarities
    Contains 1 bHLH (basic helix-loop-helix) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • bHLHc12 antibody
      • Class C basic helix loop helix protein 12 antibody
      • Class C basic helix-loop-helix protein 12 antibody
      • MAD 4 antibody
      • MAD4 antibody
      • Mad4 homolog antibody
      • MAD4_HUMAN antibody
      • Max associated protein 4 antibody
      • MAX dimerization protein 4 antibody
      • Max dimerizer 4 antibody
      • Max interacting transcriptional repressor MAD4 antibody
      • Max-associated protein 4 antibody
      • Max-interacting transcriptional repressor MAD4 antibody
      • MST 149 antibody
      • MST149 antibody
      • MSTP 149 antibody
      • MSTP149 antibody
      • MXD 4 antibody
      • MXD4 antibody
      see all

    Images

    • Western blot - Anti-MAD4 antibody (ab15841)
      Western blot - Anti-MAD4 antibody (ab15841)
      Anti-MAD4 antibody (ab15841) at 0.5 µg/ml + Lysates prepared from human kidney tissue at 35 µg

      Predicted band size: 23 kDa



      35 µg of human kidney lysate in RIPA buffer was analyzed by anti-MAD4 antibody (ab15841).  Primary antibody was incubated at 0.5 µg/ml for one hour. 
      Detection: chemiluminescence.

    References

    ab15841 has not yet been referenced specifically in any publications.

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