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Goat polyclonal to MAD4
The appearance of the 22 kDa band was blocked by incubation with the immunising peptide.
, corresponding to C terminal amino acids 197-209 of Human MAD4.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 0.5mg/ml Tris saline. pH 7.3
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 23 kDa).
Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.
Contains 1 bHLH (basic helix-loop-helix) domain.
Information by UniProt
Class C basic helix loop helix protein 12 antibody
Class C basic helix-loop-helix protein 12 antibody
Western blot - Anti-MAD4 antibody (ab15841)
Anti-MAD4 antibody (ab15841) at 0.5 µg/ml + Lysates prepared from human kidney tissue at 35 µg
Predicted band size:
µg of human kidney lysate in RIPA buffer was analyzed by anti-MAD4 antibody (ab15841). Primary antibody was incubated at 0.5 µg/ml for one hour. Detection: chemiluminescence.
has not yet been referenced specifically in any publications.
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