Overview

  • Product nameAnti-MAD4 antibody
    See all MAD4 primary antibodies
  • Description
    Rabbit polyclonal to MAD4
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Cow, Dog
  • Immunogen

    A region within synthetic peptide: DFAREKTKAA GLVRKAPNNR SSHNELEKHR RAKLRLYLEQ LKQLVPLGPD, corresponding to N terminal amino acids 36-85 of Human MAD4

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab66367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 - 2.5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • FunctionTranscriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.
  • Sequence similaritiesContains 1 bHLH (basic helix-loop-helix) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHc12 antibody
    • Class C basic helix loop helix protein 12 antibody
    • Class C basic helix-loop-helix protein 12 antibody
    • MAD 4 antibody
    • MAD4 antibody
    • Mad4 homolog antibody
    • MAD4_HUMAN antibody
    • Max associated protein 4 antibody
    • MAX dimerization protein 4 antibody
    • Max dimerizer 4 antibody
    • Max interacting transcriptional repressor MAD4 antibody
    • Max-associated protein 4 antibody
    • Max-interacting transcriptional repressor MAD4 antibody
    • MST 149 antibody
    • MST149 antibody
    • MSTP 149 antibody
    • MSTP149 antibody
    • MXD 4 antibody
    • MXD4 antibody
    see all

Anti-MAD4 antibody images

  • All lanes : Anti-MAD4 antibody (ab66367) at 0.25 µg/ml (in 5% skim milk / PBS buffer)

    Lane 1 : molecular marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 23 kDa
    Observed band size : 30,48 kDa (why is the actual band size different from the predicted?)

References for Anti-MAD4 antibody (ab66367)

ab66367 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Primary keratinocytes from skin epithelium)
Gel Running Conditions Reduced Denaturing
Loading amount 50 µg
Specification Primary keratinocytes from skin epithelium
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Feb 03 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"