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Recombinant soluble MAdCAM-1-fc fusion protein (Human).
Clone 314G8 reacts with the ligand binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognised by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM1 with alpha 4 beta 7 (see reference 1).
Our Abpromise guarantee covers the use of ab34247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/100.
Use 10µl of the suggested working dilution to label 106 cells in 100µl.
|IHC-P||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 43 kDa).|
|Functional Studies||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|