Anti-MAdCAM1 antibody [314G8] (ab34247)
Key features and details
- Mouse monoclonal [314G8] to MAdCAM1
- Suitable for: ELISA, IHC-Fr, IHC-P, Functional Studies, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-MAdCAM1 antibody [314G8]
See all MAdCAM1 primary antibodies -
Description
Mouse monoclonal [314G8] to MAdCAM1 -
Host species
Mouse -
Tested applications
Suitable for: ELISA, IHC-Fr, IHC-P, Functional Studies, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Fusion protein corresponding to Human MAdCAM1.
Database link: Q13477 -
General notes
Clone 314G8 reacts with the ligand binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognised by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM1 with alpha 4 beta 7 (see reference 1).
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
This antibody was purified from tissue culture supernatant. -
Primary antibody notes
Clone 314G8 reacts with the ligand binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognised by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM1 with alpha 4 beta 7 (see reference 1). -
Clonality
Monoclonal -
Clone number
314G8 -
Myeloma
P3x63-Ag8.653 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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Functional Studies |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Functional Studies
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. |
Target
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Function
Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding. -
Tissue specificity
Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes. -
Sequence similarities
Contains 2 Ig-like (immunoglobulin-like) domains. -
Post-translational
modificationsThe Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8174 Human
- Omim: 102670 Human
- SwissProt: Q13477 Human
- Unigene: 102598 Human
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Alternative names
- Addressin mucosal antibody
- hMAdCAM 1 antibody
- hMAdCAM-1 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab34247 has been referenced in 1 publication.
- Allavena R et al. CNS elevation of vascular and not mucosal addressin cell adhesion molecules in patients with multiple sclerosis. Am J Pathol 176:556-62 (2010). IHC-Fr ; Human . PubMed: 20035048