Mouse monoclonal [314G8] to MAdCAM1 (Phycoerythrin)
ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
Specificityab58430 recognises human mucosal addressin cell adhesion molecule 1 (MAdCAM1). MAdCAM1 is expressed on high endothelial venules of Peyer's
patches and mesenteric lymph nodes. MAdCAM1 expression has also been reported in the gut lamina propria but clone 314G8 does not recognise MAdCAM1 in these tissues. Clone 314G8 reacts with the ligand-binding
first Ig domain and reports suggest that a splice variant exists in the gut which is not recognised by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM1 with alpha 4 beta 7 (in press).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use neat - 1/10. Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
Tissue specificityHighly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.