Overview

  • Product nameAnti-MAdCAM1 antibody [314G8] (Phycoerythrin)
    See all MAdCAM1 primary antibodies
  • Description
    Mouse monoclonal [314G8] to MAdCAM1 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Specificityab58430 recognises human mucosal addressin cell adhesion molecule 1 (MAdCAM1). MAdCAM1 is expressed on high endothelial venules of Peyer's patches and mesenteric lymph nodes. MAdCAM1 expression has also been reported in the gut lamina propria but clone 314G8 does not recognise MAdCAM1 in these tissues. Clone 314G8 reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognised by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM1 with alpha 4 beta 7 (in press).
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant soluble MAdCAM1 fc fusion protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab58430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: Use neat - 1/10. Use 10ul of the suggested working dilution to label 106 cells in 100ul.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
    • Tissue specificityHighly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.
    • Sequence similaritiesContains 2 Ig-like (immunoglobulin-like) domains.
    • Post-translational
      modifications
      The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Addressin mucosal antibody
      • hMAdCAM 1 antibody
      • hMAdCAM-1 antibody
      • MACAM1 antibody
      • MADCA_HUMAN antibody
      • MAdCAM 1 antibody
      • MAdCAM-1 antibody
      • Madcam1 antibody
      • Mucosal addressin cell adhesion molecule 1 antibody
      • Mucosal vascular addressin cell adhesion molecule 1 antibody
      see all

    References for Anti-MAdCAM1 antibody [314G8] (Phycoerythrin) (ab58430)

    This product has been referenced in:
    • Dianzani C  et al. B7h triggering inhibits umbilical vascular endothelial cell adhesiveness to tumor cell lines and polymorphonuclear cells. J Immunol 185:3970-9 (2010). Flow Cyt ; Human . Read more (PubMed: 20817864) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"