Anti-MAdCAM1 antibody [AP-MAB0842] (ab80680)


  • Product nameAnti-MAdCAM1 antibody [AP-MAB0842]
    See all MAdCAM1 primary antibodies
  • Description
    Rat monoclonal [AP-MAB0842] to MAdCAM1
  • Tested applicationsSuitable for: IHC-P, Inhibition Assay, IP, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse Endothelial cells

  • General notes
    Endotoxin Level: Extremely low level of LPS (< 0.002EU/µg IgG).

    ab80680 was produced by in vitro culture, from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat).
    ab80680 is useful for reducing T cell mediated inflammation in gastrointestinal diseases.



Our Abpromise guarantee covers the use of ab80680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Inhibition Assay Use at an assay dependent concentration.

ab80680 is useful for blocking the interaction of MAdCAM1 with its counter receptor.

IP 1/50 - 1/200.
IHC-Fr 1/50 - 1/200.
Flow Cyt 1/200 - 1/400.


  • FunctionCell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
  • Tissue specificityHighly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.
  • Sequence similaritiesContains 2 Ig-like (immunoglobulin-like) domains.
  • Post-translational
    The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Addressin mucosal antibody
    • hMAdCAM 1 antibody
    • hMAdCAM-1 antibody
    • MACAM1 antibody
    • MADCA_HUMAN antibody
    • MAdCAM 1 antibody
    • MAdCAM-1 antibody
    • Madcam1 antibody
    • Mucosal addressin cell adhesion molecule 1 antibody
    • Mucosal vascular addressin cell adhesion molecule 1 antibody
    see all

Anti-MAdCAM1 antibody [AP-MAB0842] images

  • ab80680 staining MAdCAM1 in mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked for 15 minutes at room temperature; antigen retrieval was by heat mediation in citrate, pH 6.0. Samples were incubated with primary antibody (1/100 in TBS + 1% BSA) for 30 minutes. An undiluted HRP-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-MAdCAM1 antibody [AP-MAB0842] (ab80680)

ab80680 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step (agent) for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Sample Mouse Tissue sections (Colon)
Specification Colon
Permeabilization No
Fixative Formaldehyde

Mr. Alex Van Engelenburg

Verified customer

Submitted Jul 28 2014