Anti-MAdCAM1 antibody [MECA-367] (Biotin) (ab25021)

Overview

  • Product nameAnti-MAdCAM1 antibody [MECA-367] (Biotin)
    See all MAdCAM1 primary antibodies
  • Description
    Rat monoclonal [MECA-367] to MAdCAM1 (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: Other, Flow Cyt, IHC-Fr, Blocking, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Endothelial cells isolated from BALB/c mouse mesenteric and peripheral lymph nodes.

  • Epitopeab25021 reacts with an epitope located in the first domain of MAdCAM1.
  • General notes


    ab25021 blocks MAdCAM-1-dependent binding in vitro and lymphocyte homing to Peyer’s patch HEV In vivo.

Properties

Applications

Our Abpromise guarantee covers the use of ab25021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Other Use at an assay dependent concentration.
AP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab18445-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use a concentration of 1 µg/ml.
Blocking Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.

Target

  • FunctionCell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
  • Tissue specificityHighly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.
  • Sequence similaritiesContains 2 Ig-like (immunoglobulin-like) domains.
  • Post-translational
    modifications
    The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Addressin mucosal antibody
    • hMAdCAM 1 antibody
    • hMAdCAM-1 antibody
    • MACAM1 antibody
    • MADCA_HUMAN antibody
    • MAdCAM 1 antibody
    • MAdCAM-1 antibody
    • Madcam1 antibody
    • Mucosal addressin cell adhesion molecule 1 antibody
    • Mucosal vascular addressin cell adhesion molecule 1 antibody
    see all

References for Anti-MAdCAM1 antibody [MECA-367] (Biotin) (ab25021)

ab25021 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"