Anti-MAdCAM1 antibody [MECA-367] (FITC) (ab24941)

Overview

  • Product nameAnti-MAdCAM1 antibody [MECA-367] (FITC)
    See all MAdCAM1 primary antibodies
  • Description
    Rat monoclonal [MECA-367] to MAdCAM1 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: Blocking, Flow Cyt, IHC-Fr, IP, WB, Othermore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation: Endothelial cells isolated from BALB/c mouse mesenteric and peripheral lymph nodes.

  • EpitopeThis antibody reacts with an epitope located in the first domain of MAdCAM1.

Properties

Applications

Our Abpromise guarantee covers the use of ab24941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking
Flow Cyt
IHC-Fr
IP
WB
AP
Other
  • Application notesAP: Use at an assay dependent dilution.
    BL: Use at an assay dependent dilution.
    Note: This antibody is useful for in vitro cellular binding blocking, and for blocking lymphocyte homing to Peyers patch HEV in vivo.
    Flow Cyt: Use at an assay dependent dilution.
    IHC-Fr: Use at a concentration of 1 µg/ml.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 44 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
    • Tissue specificityHighly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.
    • Sequence similaritiesContains 2 Ig-like (immunoglobulin-like) domains.
    • Post-translational
      modifications
      The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Addressin mucosal antibody
      • hMAdCAM 1 antibody
      • hMAdCAM-1 antibody
      • MACAM1 antibody
      • MADCA_HUMAN antibody
      • MAdCAM 1 antibody
      • MAdCAM-1 antibody
      • Madcam1 antibody
      • Mucosal addressin cell adhesion molecule 1 antibody
      • Mucosal vascular addressin cell adhesion molecule 1 antibody
      see all

    References for Anti-MAdCAM1 antibody [MECA-367] (FITC) (ab24941)

    This product has been referenced in:
    • Hwang IY  et al. The loss of Gnai2 and Gnai3 in B cells eliminates B lymphocyte compartments and leads to a hyper-IgM like syndrome. PLoS One 8:e72596 (2013). IHC-Fr ; Mouse . Read more (PubMed: 23977324) »

    See 1 Publication for this product

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